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Plasmid#144958PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM-Venus
Plasmid#164786PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Oplophorus gracilirostris, Human)
UseTagsNanoLuc and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLacO-ISce1
Plasmid#58505Purposeprovides 256 Lac operator repeats adjacent to the ISce1 restriction siteDepositorInsertLac repeats adjacent to 6 copies of the ISce1 restriction site
UseTagsExpressionBacterialMutationPromoterno promoterAvailable sinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT374
Plasmid#125615PurposeExpresses evoFERNY-BE4max-NG in mammalian cellsDepositorInsertevoFERNY-BE4max-NG
UseTagsExpressionMammalianMutationH102P D104NPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-shNTC
Plasmid#217638PurposeDox inducible shRNA non targeting control shRNADepositorInsertshNTC
UseRNAiTagsExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 WT
Plasmid#12301DepositorInsertSTAT1 (STAT1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLX_EFS-Cre_ppt-del
Plasmid#162073PurposeCompact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEFS (part of insert)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cas9-T2A-TdT
Plasmid#126424PurposeExpresses Cas9-T2A-TdT in mammalian cells. (pTBL209)DepositorInsertCas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1339
Plasmid#119271Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-mCh
Plasmid#120169PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)DepositorInsertkinesin family member C1 (KIFC1 Human)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4)
Plasmid#17446Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, HygroDepositorHas ServiceLentiviral PrepInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Arl6ip1-mEmerald
Plasmid#186965PurposeExpress Arl6ip1 with C-terminus mEmeraldDepositorInsertARL6IP1 (ARL6IP1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pFL
Plasmid#110739PurposePlasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-mut (super repressor)
Plasmid#15291DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationS32A/S36A: “super-repressor” allele (resistant to…PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-EGFP:LIMD1
Plasmid#108230PurposeLentiviral vector for Tet-inducible LIMD1 expressionDepositorInsertLIMD1 (LIMD1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTet/ON Minimal CMV promoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTX-F
Plasmid#17438PurposeExpression of UTX in mammalian cellsDepositorInsertUTX (KDM6A Human)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM-W
Plasmid#211701PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Blast)
Plasmid#80921PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only