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pCMV-pOTC-GFP

Plasmid

#205724

Purpose

Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPEPT1

Plasmid

#195001

Purpose

Production of peptides in E. coli as GB1 fusion

Depositor

Marko Hyvönen

Article

Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

Type

Empty backbone

Use

Tags

His8-tag and StrepTagII-GB1

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b/GVcl 1-1066 /*TBM

Plasmid

#162781

Purpose

amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II

Depositor

Laura Lafon-Hughes

Article

et al PeerJ

Insert

Gallus gallus vinculin (VCL Chicken)

Use

Tags

Expression

Bacterial

Mutation

changed Gly 454 to Val

Promoter

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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EMX1*_sgRNA

Plasmid

#100558

Purpose

Expresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pME-Fv2E-flt4-ns

Plasmid

#67695

Purpose

middle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4

Use

Tags

Expression

Mutation

no stop codon; D1302E (see depositor's comme…

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE_puro

Plasmid

#118692

Purpose

Empty vector to express overexpression cDNA

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LRCherry2.1

Plasmid

#108099

Purpose

Lentiviral expression plasmid of sgRNA with mCherry

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDY1243-Fluorescent RADARS

Plasmid

#182540

Purpose

Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

mNeon

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-GFP

Plasmid

#171123

Purpose

Doxycycline inducible expression of GFP

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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LRG2.1

Plasmid

#108098

Purpose

Lentiviral expression plasmid of sgRNA with GFP

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-luciferase-P2A-Neo

Plasmid

#105621

Purpose

3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Use

Tags

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo

Plasmid

#108101

Purpose

Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti_MS2_P65_HSF1_2A_puro

Plasmid

#118699

Purpose

To express MS2-P65-HSF1

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA_MS2_neo

Plasmid

#118650

Purpose

To clone sgRNA for activation dCas9

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Use

Tags

Expression

Mammalian

Mutation

R188Q in FLuc

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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sgTrack-GFP

Plasmid

#114012

Purpose

Empty sgRNA expression vector with co-expression of TurboGFP reporter

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only