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Plasmid#211831PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.DepositorInsertmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas9_P2A_T4pol
Plasmid#190146PurposeExpresses SpCas9 and T4polDepositorInsertSpCas9_P2A_T4pol
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64
Plasmid#203349PurposeTemplate for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertmGreenLantern
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 dFE
Plasmid#190137PurposeExpresses SpCas9-T4pol (dFE)DepositorInsertSpCas9-T4pol (dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-ATP
Plasmid#225247PurposeBacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-glucose
Plasmid#225252PurposeBacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucoseDepositorInsertqmTQ2-glucose
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-citrate
Plasmid#225251PurposeBacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrateDepositorInsertqmTQ2-citrate
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-cAMP
Plasmid#225249PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
LZ3 dFE
Plasmid#190144PurposeExpresses LZ3Cas9-T4pol (LZ3 dFE)DepositorInsertLZ3Cas9-T4pol (LZ3 dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9_P2A_POLB
Plasmid#190145PurposeExpresses SpCas9 and POLBDepositorInsertSpCas9_P2A_POLB
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64
Plasmid#203348PurposeTemplate for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertEnhanced Green Fluorescent Protein
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDM4
Plasmid#49796PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64
Plasmid#203350PurposeTemplate for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertNano Luciferase
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
C035
Plasmid#174341PurposeTemplate for making Rluc mRNA reporter with pA120.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
C098
Plasmid#174345PurposeTemplate for making Nluc mRNA reporter with pA139.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C255
Plasmid#174352PurposeTemplate for making mRNA for overexpressing eGFP (C-FLAG+HA) in frog oocytesDepositorInserteGFP
UseIn vitro transcription templateTagsFLAG and HAExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6
Plasmid#49795PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
C034
Plasmid#174340PurposeTemplate for making Rluc mRNA reporter with pA98.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only