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Showing: 261 - 280 of 8352 results

  3. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60226-plasmid-map-sequence-id-176980

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  9. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid
    #202199
    Purpose
    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Has Service
    AAV1 and AAV5
    Insert
    stChrimsonR, PdCO
    Use
    AAV and Cre/Lox
    Tags
    EGFP and Rho1D4
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202199-plasmid-map-sequence-id-397078

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  10. pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

    Plasmid
    #202198
    Purpose
    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Insert
    stChrimsonR, PdCO
    Use
    AAV
    Tags
    EGFP and Rho1D4
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIα minimal promotor (0.4kb)
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202198-plasmid-map-sequence-id-397077

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  11. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid
    #183078
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    V600E (T>A), S607S (TCC>AGT)
    Promoter
    None for primary insert. PGK for puromycin resist…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183078-plasmid-map-sequence-id-363420

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  12. pUC19-link-mVenus-FLAG:Kan

    Plasmid
    #211830
    Purpose
    Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.
    Depositor
    Peng Chen
    Insert
    mVenus-FLAG-Kan
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Available since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211830-plasmid-map-sequence-id-418060

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  13. pUC19-link-PAmCherry-FLAG:cat

    Plasmid
    #211832
    Purpose
    Plasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.
    Depositor
    Peng Chen
    Insert
    PAmCherry-FLAG-cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211832-plasmid-map-sequence-id-426747

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  14. pUC19-link-mCherry-FLAG:cat

    Plasmid
    #211831
    Purpose
    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.
    Depositor
    Peng Chen
    Insert
    mCherry-FLAG-cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Available since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211831-plasmid-map-sequence-id-418058

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  15. pUC19-link-sfGFP-FLAG:Kan

    Plasmid
    #211833
    Purpose
    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.
    Depositor
    Peng Chen
    Insert
    sfGFP-FLAG-Kan
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Available since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211833-plasmid-map-sequence-id-418068

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  16. pUC19-link-PAGFP-FLAG:Kan

    Plasmid
    #211834
    Purpose
    Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.
    Depositor
    Peng Chen
    Insert
    PAGFP-FLAG-Kan
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Available since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211834-plasmid-map-sequence-id-418061

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  18. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

    Plasmid
    #203349
    Purpose
    Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    mGreenLantern
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203349-plasmid-map-sequence-id-399147

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  19. SpCas9 dFE

    Plasmid
    #190137
    Purpose
    Expresses SpCas9-T4pol (dFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    SpCas9-T4pol (dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190137-plasmid-map-sequence-id-382840

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Showing: 261 - 280 of 8352 results