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pMito-LSSmKate1-N1

Plasmid

#31878

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmKate2-N1

Plasmid

#31879

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pLacO-pEF1α-RFP

Plasmid

#179507

Purpose

Expresses monomeric RFP in mammalian cells under a EF1α promoter

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

monomeric RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NPY-ClopHensor

Plasmid

#25939

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)

Use

Tags

NPY and Strep-II

Expression

Mammalian

Mutation

Neuropeptide Y localization signal

Promoter

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pCI AscI MR1 res

Plasmid

#214752

Purpose

expresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cells

Depositor

David Lewinsohn

Article

Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

Insert

MHC class I-related protein 1 (MR1 Human)

Use

Tags

IRES eGFP

Expression

Mammalian

Mutation

silent mutations to prevent editing by CRISPR/Cas…

Promoter

CMV

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pNSEN-d2

Plasmid

#59763

Purpose

Expresses nuclear EGFP and Tomato in mammalian cells

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

Inserts

d2-EGFP
Tomato

Use

Tags

3X NLS and PEST

Expression

Mammalian

Mutation

Promoter

CMV promoter and SV40 promoter

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Cerulean

Plasmid

#15214

Depositor

Dave Piston

Article

Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

Insert

monomeric Cerulean

Use

Tags

Expression

Mammalian

Mutation

A206K

Promoter

Available since

June 22, 2007

Availability

Academic Institutions and Nonprofits only

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GPI-mCherry2

Plasmid

#182869

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

GPI-mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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GPI-mApple

Plasmid

#182868

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

GPI-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GPI-mEYFP

Plasmid

#182867

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

GPI-mEYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI AscI MR1 R9H res

Plasmid

#214753

Purpose

expresses human MR1 R9H resistant to CRISPR editing with a specific sgRNA in mammalian cells

Depositor

David Lewinsohn

Article

Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

Insert

MHC class I-related protein 1 (MR1 Human)

Use

Tags

IRES eGFP

Expression

Mammalian

Mutation

silent mutations to prevent editing by CRISPR/Cas…

Promoter

CMV

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mGreenLantern

Plasmid

#197928

Purpose

Monomer expression vector

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NeonGreen

Plasmid

#197924

Purpose

Monomer expression vector

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

NeonGreen

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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Gamillus

Plasmid

#197926

Purpose

Monomer expression vector

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

Gamillus

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOCC65

Plasmid

#118865

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease, mGFP

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC61

Plasmid

#118877

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette

Use

Tags

mGFP, HIS6, cleavable with 3C protease

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOCC139

Plasmid

#118872

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease and mGFP

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GPI-Gamillus

Plasmid

#197914

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GPI-mNeonGreen

Plasmid

#197912

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GPI-mGreenLantern

Plasmid

#197916

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge