GPI-mApple
(Plasmid
#182868)
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PurposeMonomer expression vector with plasma membrane localization signal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGPI-mApple
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SpeciesOther
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GPI-mApple was a gift from Salvatore Chiantia (Addgene plasmid # 182868 ; http://n2t.net/addgene:182868 ; RRID:Addgene_182868) -
For your References section:
Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection. Dunsing V, Petrich A, Chiantia S. Elife. 2021 Sep 8;10. pii: 69687. doi: 10.7554/eLife.69687. 10.7554/eLife.69687 PubMed 34494547