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Showing: 261 - 280 of 302 results

  6. (SUMO)10-(SIM)5

    Plasmid
    #126866
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126866-plasmid-map-sequence-id-263307

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  7. (SUMO)5-(SIM)10

    Plasmid
    #126923
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    internal BamHI site between (SUMO)5 and (SIM)10
    Promoter
    tac
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126923-plasmid-map-sequence-id-255302

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  8. RFP-(SUMO)10-(SIM)6

    Plasmid
    #122026
    Purpose
    Mammalian expression plasmid for multivalent scaffold of (SUMO)10-(SIM)6 biomolecular condensates to recruit SIM-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    10 copies of human SUMO3 and 6 repeats of human SUMO interactive motif (SIM) from PIASx separated by (GGS)4 linker (SUMO3, PIAS2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    122026-plasmid-map-sequence-id-255492

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  9. RFP-(SUMO)6-(SIM)10

    Plasmid
    #122027
    Purpose
    Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    6 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2, SUMO3 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122027-plasmid-map-sequence-id-434238

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  11. TRIPZ-bioGEF-SUMO1nc-PURO

    Plasmid
    #208042
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208042-plasmid-map-sequence-id-410263

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  12. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Expression
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

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  14. pcDNA3-His-SUMO3

    Plasmid
    #159325
    Purpose
    Mammalian cell expression of mouse His-SUMO3
    Depositor
    Jianjun Zhao
    Insert
    His-SUMO3 (Sumo3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159325-plasmid-map-sequence-id-311868

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  15. pLV V5-Senp2

    Plasmid
    #175118
    Purpose
    Lentiviral expression of V5-tagged mouse Senp2
    Depositor
    Larry Gerace
    Insert
    Senp2 (Senp2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175118-plasmid-map-sequence-id-351807

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  17. pCoofy63

    Plasmid
    #122015
    Purpose
    Baculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122015-plasmid-map-sequence-id-243586

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  19. pCoofy65

    Plasmid
    #125176
    Purpose
    Mammalian vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    125176-plasmid-map-sequence-id-243578

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  20. pCoofy64

    Plasmid
    #122016
    Purpose
    Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122016-plasmid-map-sequence-id-243594

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Showing: 261 - 280 of 302 results