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pUC21-deltaBB

Plasmid

#51657

Purpose

Bacterial cloning vector based on pUC21 (Amp)

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pMB38-HF380deltaMTBD

Plasmid

#37333

Depositor

Takahide Kon

Article

Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

Insert

Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)

Use

Dictyostelium expression

Tags

His6 and FLAG tag

Expression

Mutation

deleted amino acids 1-1387 and replaced amino aci…

Promoter

TRE-Pmin

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only
pLG-deltaA

Plasmid

#33151

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Use

Tags

LacZ

Expression

Yeast

Mutation

The RP51A sequence was modified by digestion with…

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pRSF-KpDcr1delta2d

Plasmid

#32028

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-260)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pYES2.1-ScDcr1deltaC

Plasmid

#32037

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

Drc1(17-356)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
T4 Lysozyme R8-delta WT*

Plasmid

#18222

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Use

Tags

Expression

Bacterial

Mutation

R8-delta WT*

Promoter

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only
pESdelta 799

Plasmid

#13042

Depositor

Peggy Hsieh

Article

Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

Insert

MutS

Use

Tags

His6

Expression

Bacterial

Mutation

C-terminal deletion from aa 800 through 853.

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCMV delta R8.2

Plasmid

#12263

Purpose

First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

Depositor

Didier Trono

Insert

HIV-1 GAG/POL, Tat and Rev

Use

Lentiviral; Packaging

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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DRH002_scAAV-hSyn-delta_iCre-HA

Plasmid

#225087

Purpose

Expression of inactive delta-iCre (control) with an HA tag in neurons driven by human synapsin promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

delta_iCre

Use

AAV and Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-R2L3-IRESpacDeltaTKpA

Plasmid

#139535

Purpose

Provides IRES-pacDeltaTK-pA cassette on Gateway entry vector

Depositor

Minoru Ko

Insert

PacTK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pY003 (pFnCpf1_delta Cas)

Plasmid

#69974

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Cas genes are deleted from locus

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAKalpha_deltaCTD

Plasmid

#141196

Purpose

Expresses Strep-HA-ZAKalpha_deltaCTD in mammalian cells, can be used to make inducible cell lines

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Use

Tags

Strep, HA

Expression

Mammalian

Mutation

deletion of last 27 amino acids

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCIBN(deltaNLS)-pmGFP

Plasmid

#26867

Purpose

Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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myrAkt delta4-129

Plasmid

#10841

Depositor

Richard Roth

Article

Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

Insert

Akt (AKT1 Human)

Use

Tags

HA and myr

Expression

Mammalian

Mutation

Deletion of PH domain (aa4-129)

Promoter

SV40

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Promoter

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Use

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pUC19 PKC delta

Plasmid

#8427

Depositor

Frederic Mushinski

Article

Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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TagBFP2-C1-SACM1LdeltaTMD-Fis1tail

Plasmid

#220078

Purpose

SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-Fis1tail (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

deltaTMD

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-1-SAC1deltaTMD-Fis1tail

Plasmid

#220077

Purpose

SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-Fis1tail (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

deltaTMD

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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