Addgene

We narrowed to 691 results for: KO

Showing: 261 - 280 of 691 results

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pGL6

Plasmid

#48746

Purpose

Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region (-1128 to +2129)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CWB-pre-eGRASP(p32)

Plasmid

#111598

Purpose

An AAV vector that expresses green pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

green pre-eGRASP(p32)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-cyan pre-eGRASP(p30)

Plasmid

#111586

Purpose

An AAV vector that expresses cyan pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p30)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-yellow pre-eGRASP(p30)

Plasmid

#111587

Purpose

An AAV vector that expresses yellow pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p30)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-yellow pre-eGRASP(p30)

Plasmid

#111588

Purpose

An AAV vector that expresses yellow pre-eGRASP(p30) under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p30)

Use

AAV

Tags

Expression

Mutation

Promoter

tetO

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-1xHA CARM1 WT

Plasmid

#81118

Purpose

mammalian expression of CARM1 WT

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

CARM1 (Carm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-CWB-cyan pre-eGRASP(p32)

Plasmid

#111579

Purpose

An AAV vector that expresses cyan pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-yellow pre-eGRASP(p32)

Plasmid

#111580

Purpose

An AAV vector that expresses yellow pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p32)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-yellow pre-eGRASP(p32)

Plasmid

#111582

Purpose

An AAV vector that expresses yellow pre-eGRASP(p32) under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p32)

Use

AAV

Tags

Expression

Mutation

Promoter

tetO

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-pre-eGRASP(p30)

Plasmid

#111597

Purpose

An AAV vector that expresses green pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

green pre-eGRASP(p30)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pNEXdelta-ApAF

Plasmid

#13334

Depositor

Bong-Kiun Kaang

Article

Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

Insert

Aplysia activating factor

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
pGL2-Skp2 promoter-luciferase

Plasmid

#81119

Purpose

Reporter activity of SKP2 gene promoter

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (Skp2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mutation

contains the region from 1_kb upstream of transcr…

Promoter

SKP2

Available since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only
p.UTA.2.0-GL-2

Plasmid

#82440

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

Luciferase

Use

Tags

Expression

Mutation

The complementary region for GL2 siRNA control wa…

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR 19b-3p

Plasmid

#82442

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-19-3p

Use

Tags

Expression

Mutation

Complementary region for hsa-miR-19-3p as sensor

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG MKRN1 S109A

Plasmid

#78774

Purpose

To overexpress MKRN1 S109A in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

MKRN1 (MKRN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S109A_mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG MKRN1 S109D

Plasmid

#78775

Purpose

To overexpress MKRN1 S109D in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

MKRN1 (MKRN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S109D_mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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UASp-Fz4

Plasmid

#52400

Purpose

Over-expression of Drosophila Frizzled 4

Depositor

Roel Nusse

Article

McElwain et al PLoS One. 2011;6(11):e26993. doi: 10.137

Insert

Drosophila Frizzled 4 (fz4 Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

UAS

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pBR_attB(bxb)_lox

Plasmid

#183762

Purpose

Bxb1-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG PTEN

Plasmid

#78777

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-cyan pre-eGRASP(p32)

Plasmid

#111589

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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