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Plasmid#184496PurposeExpresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteriaDepositorInsertiMbody
UseTags3xFLAG, 6xHis, and pelB leaderExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-tTA-ER-C3
Plasmid#134568PurposeExpresses a YFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion proteinDepositorInserttTA-ER
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63-GG
Plasmid#208015Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-α5last
Plasmid#160270PurposeExpresses a concatemeric construct of a α4:β2:α5 (2:2:1) nAChRDepositorInsertα5last concatemer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAC212
Plasmid#187912Purposepsac212-crispey-bar-ade2KODepositorInsertade2 KO CRISPEY cassette
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA23-GG
Plasmid#208013Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYC.Taq.1Amb
Plasmid#102794PurposeExpresses Taq DNA polymerases with a premature stop codonDepositorInsertTaq DNA polymerase
UseTagsExpressionBacterialMutationW168Stop; *(see below)PromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA13-GG
Plasmid#208002Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBPGUw-HACK-G4>QF2
Plasmid#80277PurposeGAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
T1ACR1_EYFP_pcDNA3.1
Plasmid#161027PurposeExpresses strongly red-shifted T1ACR1 in mammalian cellsDepositorInsertT1ACR1
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SaMscL (CDelta26)
Plasmid#79027PurposeStaphylococcus aureus MscL with C Terminal Truncation of 26 amino acidsDepositorInsertMscL
UseTags6x Histidine TagExpressionMutationStop codon placed before 26 amino acids at C Term…PromoterT7 PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUdOm2.2
Plasmid#210279PurposeEmpty backbone with ampicillin resistance for high expression in mammalian cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdOm2.1
Plasmid#210278PurposeEmpty backbone with ampicillin resistance for high expression in mammalian cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-STReTCh-RGD
Plasmid#216532PurposeExpresses HaloTag-STReTCh-RGD fusion protein in bacteriaDepositorInsertHaloTag-STReTCh-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA43-GG
Plasmid#208014Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046487
Plasmid#149027PurposeYeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWS1078
Plasmid#123027PurposeZ3E promoterDepositorInsertpZ3 [2]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 4B
Plasmid#102713PurposeProvides the End-to-End linker 4B which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKB236
Plasmid#170027PurposeEncodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
TCP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only