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Plasmid#176236PurposePlasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176237PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176238PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBS-f1(+)-ACTB-iTagRFP-T
Plasmid#115915Purposedonor to insert iTagRFP-T at the ACTB locus in human cellsDepositorInsertACTB-TagRFP-T (ACTB Human)
UseDonor templateTagsTagRFP-TExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)
Plasmid#122450PurposeExpression of inositol 1,4,5-trisphosphate 3-kinase ADepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
UseTagsbeta-galactosidaseExpressionBacterialMutationcoding sequence from V66 till stop codonPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
ITPKC gRNA (BRDN0001147258)
Plasmid#77650Purpose3rd generation lentiviral gRNA plasmid targeting human ITPKCDepositorInsertITPKC (ITPKC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ITPKC gRNA (BRDN0001148039)
Plasmid#77651Purpose3rd generation lentiviral gRNA plasmid targeting human ITPKCDepositorInsertITPKC (ITPKC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ITPKC gRNA (BRDN0001149433)
Plasmid#77652Purpose3rd generation lentiviral gRNA plasmid targeting human ITPKCDepositorInsertITPKC (ITPKC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ITPKC gRNA (BRDN0001148914)
Plasmid#77653Purpose3rd generation lentiviral gRNA plasmid targeting human ITPKCDepositorInsertITPKC (ITPKC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cntn1-Fc-His
Plasmid#72065PurposeExpresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertCntn1 (Cntn1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2609
Plasmid#144135PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXA2 (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-MYO5C
Plasmid#135404PurposeExpresses GFP-tagged human MYO5C in mammalian cells.DepositorInsertMYO5C (MYO5C Human)
UseTagsEGFPExpressionMammalianMutationRelative to the GenBank MYO5C cDNA sequence repor…PromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2610
Plasmid#144136PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXA2 (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CA-RIT-NFAT1 (PGK-Puro)
Plasmid#63214PurposeConstitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection markerDepositorInsertCA-RIT-NFAT1 (Nfatc2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationAmino acids 1-3 removed; CA: PASSGSSASF mutated t…PromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-ERK2-MEK1_R4F_coexpression
Plasmid#39212DepositorUseTagsHis6 and RBSExpressionBacterialMutationMEK1(R4F) = S218E, S222D and deletion of AA32-51PromoterT7 and T7 via RBSAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-DnaJB1-PKAcat-mCherry
Plasmid#181850PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.DepositorUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPV00500 pET21GG2 His(6)-TF-BECN(1-450)
Plasmid#136684PurposeExpresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)DepositorInsertBeclin-1 (BECN1 Human)
UseTagsTrigger FactorExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Ef1a-hMGT-PGK-puro
Plasmid#172394PurposeLentiviral expression vector for human direct cardiac reprogrammingDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only