pBS-f1(+)-ACTB-iTagRFP-T
(Plasmid #115915)

• Purpose: donor to insert iTagRFP-T at the ACTB locus in human cells
• Depositing Lab: Erik Sontheimer
• Publication: Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10.1089/crispr.2022.0058. Epub 2022 Sep 7.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 2960
• Total vector size (bp): 5870
• Modifications to backbone: added homology arms and a fluorescent protein.
• Vector type: donor template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACTB-TagRFP-T
• Species: H. sapiens (human)
• Insert Size (bp): 2700
• Entrez Gene: ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
• Tag / Fusion Protein:
  • TagRFP-T

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert is based on Addgene plasmid: AICSDP-15:ACTB-mEGFP (Plasmid #87425).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TagRFP-T was inserted. Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.