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Plasmid#167616PurposeExpress TonB C18G, Q160C.DepositorInserttonB C18G, Q160C
UseTagsExpressionBacterialMutationtonB with C18G, Q160C mutations.PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1683
Plasmid#167622PurposeExpress TonB C18G, V206C.DepositorInserttonB C18G, V206C
UseTagsExpressionBacterialMutationtonB with C18G, V206C mutationsPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1362
Plasmid#167615PurposeExpress TonB C18G.DepositorInserttonB C18G
UseTagsExpressionBacterialMutationtonB with C18G mutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1684
Plasmid#167630PurposeExpress TonB C18G, R214C.DepositorInserttonB C18G, R214C
UseTagsExpressionBacterialMutationtonB with C18G, R214C mutationsPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP476
Plasmid#167594PurposeExpress TonB ∆(199-216)DepositorInserttonB ∆(199-216)
UseTagsExpressionBacterialMutationtonB ∆(199-216)PromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP372
Plasmid#167593PurposeExpress TonB frameshift of amphipathic helixDepositorInserttonB fs
UseTagsExpressionBacterialMutationtonB with frameshift of amphipathic helixPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAS9id_TRP
Plasmid#141254PurposeGalactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterGAL1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
C114m
Plasmid#121013PurposeMoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.DepositorInsertdCas9 with no bbsI or BsaI sites
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
371-C
Plasmid#97029PurposeHis6-MBP-N10-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, MBP domain, N10 linker, and TEV cleavag…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC120
Plasmid#50350PurposepUC cloning vectorDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSA54
Plasmid#35113DepositorInsertcallys5MX3
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSA49
Plasmid#35118DepositorInsertcallys5MX4
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPH 34
Plasmid#35098DepositorInsertfca1MX3
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSA52
Plasmid#35119DepositorInsertcallys5MX3
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSA50
Plasmid#35111DepositorInsertcalys5MX4
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBN42
Plasmid#35103DepositorInsertkanPR
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBSCSX
Plasmid#24072DepositorInsertCLiPS caspase-3 substrate
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag
Plasmid#208051PurposeEmpty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with FlagDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT0127
Plasmid#8728DepositorInsertyEGFP
UseTagsCodon optimized linkerExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRL055 pDESTattB_cryaa:Venus
Plasmid#213509PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only