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Plasmid#171613PurposeNLS-AsCas12f1-NLS heterologous expression plasmid for RNPDepositorInsertNLS-AsCas12f1-NLS
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-Nsp14-TEV-6His
Plasmid#154952PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp14-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS0663 pET45b(+) KraIscB-1 TwinStrep
Plasmid#176536PurposeBacterial expression of KraIscB-1-TwinStrepDepositorInsertKraIscB-1
UseTagsTwinStrepExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF6-V5-His6
Plasmid#67415PurposeExpression of hArf6 with C-term V5 and His6 tag in E. coliDepositorInsertARF6 (ARF6 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP6 (catalytic domain)
Plasmid#16359DepositorInserthuman SENP6 catalytic domain (SENP6 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 628 to 1112 catalytic domain of human …PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22b-mEB3-mCherry-6xHis
Plasmid#130985PurposeBacterial expression of mouse EB3-mCherry-6xHis for protein purification.DepositorInsertEB3-mCherry-6xHis (Mapre3 Mouse)
UseTagsmCherry-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-6His-MBP-MpAgo
Plasmid#79249PurposeThis plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expressionDepositorInsertMarinitoga piezophila Argonaute
UseCRISPRTags6XHis-tag + MBP + PreScission Cleavage SiteExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorInsertSNCA-GFP (SNCA Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-1_enzymatic-domain
Plasmid#183385PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1 (AA130-404) (Casp1 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NADP-Snifit
Plasmid#113913PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+DepositorInsertNADP-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-K128N
Plasmid#120284PurposeProduce human recombinant FGF2 K128N in DE3 cellsDepositorInsertBasic fibroblast growth factor 2 (FGF2 Synthetic, Human)
UseTags6x HisExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP8 (full length))
Plasmid#16361DepositorInserthuman SENP8 full length (SENP8 Human)
UseTagsHisExpressionBacterialMutationhuman SENP8 full length, see Depositor CommentsPromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-SNAPf
Plasmid#188941PurposeT7 expression vector for making his6- and HA3-tagged SNAP proteinDepositorInserthis6-HA3-SNAPf
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-dspB(E184Q)-6xHis
Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-alpha-synuclein-BiFC
Plasmid#87855PurposepETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisationDepositorInsertsalpha-synuclein-VC155
alpha-synuclein-VN154
UseTagsC-terminal fragment of mVenus BiFC protein and N-…ExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Histag-Cerulean (526)
Plasmid#70716PurposeExpression vector for Cerulean purificationDepositorInsertCerulean
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
UseTags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorInsertTGFa-GSY-SPM (TGFA Synthetic, Human)
UseTagsC-tag and His6ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only