pET28sumo-MDA5-Full-length
(Plasmid
#210826)
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Purposeprotein expression in ecoli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210826 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepet28-sumo
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMDA5 (IFIH1) Full Length Protein
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SpeciesH. sapiens (human)
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MutationNA
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Entrez GeneIFIH1 (a.k.a. AGS7, Hlcd, IDDM19, IMD95, MDA-5, MDA5, RLR-2, SGMRT1)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28sumo-MDA5-Full-length was a gift from Anna Pyle (Addgene plasmid # 210826 ; http://n2t.net/addgene:210826 ; RRID:Addgene_210826) -
For your References section:
Dicer-related helicase 3 forms an obligate dimer for recognizing 22G-RNA. Fitzgerald ME, Vela A, Pyle AM. Nucleic Acids Res. 2014 Apr;42(6):3919-30. doi: 10.1093/nar/gkt1383. Epub 2014 Jan 15. 10.1093/nar/gkt1383 PubMed 24435798
