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AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171594

Purpose

Strong human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Dre

Plasmid

#55633

Purpose

Expresses Dre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Inserts

mCherry
Dre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pME-mCherry-FLAG-CD59-GPI

Plasmid

#50378

Purpose

Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Article

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-Con/Fon-mCherry

Plasmid

#137132

Purpose

Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 1

Plasmid

#206992

Purpose

Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 2

Plasmid

#206993

Purpose

Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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Gal:mCherry-CRY2-uTEV1Δ(220-242)

Plasmid

#135452

Purpose

Yeast expression of uTEV1Δ fused to CRY2 and mCherry

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-CRY2-TEV1Δ(220-242)

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Gal

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)

Plasmid

#129027

Purpose

targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA cloning site

Use

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

Plasmid

#163179

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-sfGFP-kanMX (pMaM17)

Plasmid

#173452

Purpose

Contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

Insert

mCherry-sfGFP-ADH1ter

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pWPT-/GCCACC-mEGFP-IRES-mCherry

Plasmid

#49235

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Mutation

Promoter

EF1-short

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMT-sox10-NLS-BirA-2A-mCherry_Ras

Plasmid

#80063

Purpose

Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Mutation

Promoter

Sox10 promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

Plasmid

#167583

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mCherry-WPREpA

Plasmid

#177328

Purpose

Control vector for Flp-dependent DREADD constructs

Depositor

Ulrik Gether

Insert

mCherry

Use

AAV; Flp-frt

Tags

Expression

Mammalian

Mutation

Promoter

Human Synapsin-1

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

Plasmid

#177183

Purpose

plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

sgRNA targeting the PEAR-GFP plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mCherry-PA-Rac1

Plasmid

#22035

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCherry-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Tet-CMV

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pMK453 (BSD-P2A-dTAG-mCherry)

Plasmid

#214383

Purpose

dTAG-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

BSD-P2A-dTAG-mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-mCherry-WPRE-pA

Plasmid

#37083

Purpose

Cre-dependent expression of mCherry

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

mCherry

Use

AAV and Cre/Lox; Cre-on

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

Plasmid

#128268

Purpose

Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

Depositor

Stephen Royle

Article

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Inserts

MitoTrap
FKBP(III)-GBPen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and IRES

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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