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pFTK043

Plasmid

#171315

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eGFP fluorescent reporter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-neoCV

Plasmid

#73889

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

neoCV

Use

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAM5057

Plasmid

#120085

Purpose

Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

Depositor

Susan Golden

Article

Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

Insert

RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2

Plasmid

#118853

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2

Use

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Thy1-Brainbow-1.0 H

Plasmid

#18724

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

tdimer2 ; M-mEYFP ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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hSgk1_v2 Flag

Plasmid

#86040

Purpose

full length human Sgk1_i2 with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

Insert

Alternate transcript v2 of human Sgk1 (SGK1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

v2

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL30004

Plasmid

#50302

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, YFP variant of GFP (Aequorea victoria)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B28-Leum

Plasmid

#44327

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B28-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B28-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pFTK046

Plasmid

#171318

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1 fluorescent reporter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL80007

Plasmid

#50323

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mCherry variant of RFP (Discosoma sp.)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45152

Plasmid

#50263

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, cab1 (AT1g29930, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p8203 MSCV-neo C-V5 16E7

Plasmid

#193976

Purpose

HPV16 E7 expression, C-terminal V5 tag

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

HPV16 E7

Use

Retroviral

Tags

V5

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pN-PURO-PTP

Plasmid

#51711

Purpose

contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Expression

Mutation

Promoter

Available since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T1 R1276P (+minintr, KDR)

Plasmid

#119202

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 1 mini-gene (NF1 Human)

Use

Transfer vector

Tags

Expression

Mutation

R1276P

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T2 R1276P (+minintr, KDR)

Plasmid

#119203

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 2 mini-gene (NF1 Human)

Use

Transfer vector

Tags

Expression

Mutation

R1276P

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-Leum

Plasmid

#44324

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-Leum

Available since

May 23, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-ECFP-Paxillin

Plasmid

#15211

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-

Insert

paxillin (PXN Chicken)

Use

Tags

ECFP and Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pWalium-ChtVis-Tomato

Plasmid

#67756

Purpose

Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.

Depositor

Paul Adler

Article

Sobala et al Development. 2015 Nov 15;142(22):3974-81

Insert

dyl - chitin binding domain - tdTomato - dyl

Use

Tags

td-tomato

Expression

Insect

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL50007

Plasmid

#50308

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal FLAG tag

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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His-PaIct

Plasmid

#111293

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

succinyl-CoA:itaconate CoA transferase

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2021

Availability

Academic Institutions and Nonprofits only

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