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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1-S127A/S397A/S94A

Plasmid

#213586

Purpose

Doxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A, S397A, S94A

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-PR50

Plasmid

#84905

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Use

Tags

FLAG and Myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ATF4

Plasmid

#192911

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ATF4 (ATF4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-GR100

Plasmid

#84908

Purpose

induce expression of dipeptide repeat GR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GR100 (C9orf72 Synthetic)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pDY0207 ACTB atgRNA with v1 scaffold

Plasmid

#179107

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-hHelios-IRES-GFP

Plasmid

#74047

Purpose

retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Helios (IKZF2) (IKZF2 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

encodes shorter version of human Helios

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-FOXL2-cterm

Plasmid

#192893

Purpose

Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 C-term sgRNA (FOXL2 Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZBTB16

Plasmid

#192903

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ZBTB16

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ZBTB16 (ZBTB16 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-hIKAROS-IRES-GFP

Plasmid

#74046

Purpose

retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Ikaros (IKZF1) (IKZF1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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SHIP2-WT

Plasmid

#214907

Purpose

expression of the full length variants of the SHIP2

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 (INPPL1 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 puro - CRBN (#1)

Plasmid

#166240

Purpose

sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

Depositor

Günter Schneider

Article

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)-sfGFP

Plasmid

#145171

Purpose

Mammalian expression plasmid for soluble ACE2-super folder GFP fusion

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Linker GSGGSGSGG and superfolder GFP

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

Plasmid

#177120

Purpose

dCasRx mediated RNA tethering of FTO

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

dCasRx-FTO

Use

Lentiviral

Tags

HA and SV40 NLS

Expression

Mutation

dCasRX contains R239A/H244A/R858A/H863A mutations…

Promoter

Ef1a

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-WT(732)-8h

Plasmid

#154101

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (8his tag)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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TetO-dCas9-D3A

Plasmid

#78254

Purpose

Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

Depositor

Grant Challen

Article

McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

Insert

DNMT3A CD aa 598-912 (DNMT3A Human)

Use

CRISPR

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MYC

Plasmid

#192899

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of MYC

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

MYC (MYC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2 puro - CRBN (#2)

Plasmid

#166241

Purpose

sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

Depositor

Günter Schneider

Article

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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