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Plasmid#227239PurposeInducible ATP sensor for Pseudomonas putidaDepositorInsertiATPsnFR1.1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY1
Plasmid#21760DepositorInsertECFP -EYFP linked by flexible one SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
Plasmid#55613PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cellsDepositorInsert3xFLAG tagged HERC2 (WT) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationSilent mutations have been incorporated into the …PromoterAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Optimized UBE1L
Plasmid#165099Purposeecoli optimized expression of GST-fusion of human Ube1LDepositorInsertUbeE1L (UBA7 Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_Puro_sgTP53
Plasmid#187819PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53DepositorInsertsgTP53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-Talin rod-mCherry
Plasmid#133567PurposeExpresses SpyTag003-Talin rod-mCherry in mammalian cellsDepositorInsertSpyTag003-Talin rod-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_Short_V5-GFP
Plasmid#221497PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_AncBE4max_P2A_GFP
Plasmid#112100PurposeC:G-to-T:A base editingDepositorInsertAncBE4max_P2A_GFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3m
Plasmid#208702PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3m
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT18mobsacB
Plasmid#72648Purposesucrose counter-selection allelic exchange vector for Pseudomonas syringaeDepositorTypeEmpty backboneUseSucrose counter-selection allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-6xHis-SUMO-MarathonRT
Plasmid#109029PurposeGroup II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”DepositorInsertERE_01490
UseTags6xHis-SUMO tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmAmetrine-DEVD-tdTomato
Plasmid#18879PurposeCaspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomatoDepositorInsertmAmetrine-DEVD-tdTomato (CASP3 lab constructed, Human)
UseTagsExpressionMammalianMutationmAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…PromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
DDX5 WT V5
Plasmid#199576PurposeExpresses WT DDX5 cloned into pLenti puroDepositorInsertDDX5 (DDX5 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-cIAP2/pRK5
Plasmid#27973DepositorInsertCIAP2 (BIRC3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
UseTagsExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-mTert
Plasmid#162555PurposeOverexpression of TertDepositorInsertOverexpression of Tert (Tert Mouse)
UseTagsExpressionMammalianMutationNonePromoterEF1AAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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tdTomato-pBAD
Plasmid#54856PurposeLocalization: Protein Expression Vector , Excitation: 554, Emission: 581DepositorTypeEmpty backboneUseTags6xHis and tdTomatoExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-PBRM1
Plasmid#65387PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertPBRM1 (PBRM1 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH2
Plasmid#204230PurposeBacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).InserthMDH2 (MDH2 Human)
UseTags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCap-SG5-Apr
Plasmid#227579PurposeCapture plasmid with apramycin resistance and pSG5 repliconDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-CTERM-GFP
Plasmid#185770PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
Plasmid#79620PurposeExpresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1 and UGIExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL410_INS421
Plasmid#49057PurposeExpresses luciferase under control of human insulin gene promoterDepositorInserthuman INS promoter (INS Human)
UseTagsExpressionMammalianMutationPromoterINS promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term StrepII eGFP 3C
Plasmid#160683PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsStrepII eGFP 3CExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM468-EGFP
Plasmid#108434PurposeORBIT integrating plasmid for C-terminal tagging with EGFP.DepositorInsertattB-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only