-
Plasmid#31275DepositorInsertRecG-ST2-TaV-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pME-24xMBS
Plasmid#86246PurposeMiddle entry vector for generating MBS tagged RNAsDepositorTypeEmpty backboneUseUnspecifiedTagsNLS-tdMCPExpressionMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max_P2A_GFP
Plasmid#112099PurposeC:G-to-T:A base editingDepositorInsertBE4max_P2A_GFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-INSyibp-Nef
Plasmid#134593PurposeLow Phosphate inducible NefDepositorInsertHIV Nef protein
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAM-RecG-EGFP
Plasmid#31274DepositorInsertRecG-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF NEF NBP1 (P#375)
Plasmid#14657DepositorInsertNEF NBP1 (ATP6V1H Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pLentiCrispr-V2-mOrange (V2mO)
Plasmid#140206PurposeLenti Crispr/Cas9 with mOrange fluorescent markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterpELF1aAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223164Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)
Plasmid#213034Purposefusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogeneticsDepositorInsertsmNeonGreen
ISN-IE
ChRmine
UseAAVTagsGaussia luciferase signal sequenceExpressionMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA
Plasmid#218527PurposesgRNA targeting human EIF2AK2/PKRDepositorInsertEIF2AK2 (EIF2AK2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA3G-IH
Plasmid#214118PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInserttrTA3G
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-PO-MT-P2A
Plasmid#214899PurposeExpresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sitesDepositorInsertSPLICSFAST-PO-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII
Plasmid#204971PurposeSoma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.DepositorInsertraACR
UseAAVTagsmCerulean3ExpressionMutationPromoterCaMKIIAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
raACR-pmCerulean3-N1
Plasmid#204959PurposeExpression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cellsDepositorInsertraACR
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMV (+enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)
Plasmid#205098PurposeCre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogeneticsDepositorInsertsmNeonGreen
eKaz luciferase
Volvox Channelrhodopsin 1
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dSaCas9-KRAB-gRNA-TRE-blast
Plasmid#201151PurposeLentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsSadCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAExpressionMutationgRNA sequence: ATCAGTGATAGAGAACGTATGPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgMSLN
Plasmid#193588PurposeMSLN knockoutDepositorInsertsgMSLN (MSLN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.UB
Plasmid#191556PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.SFFV-E
Plasmid#191555PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.E
Plasmid#191553PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.SFFV
Plasmid#191554PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1,2,4
Plasmid#171137Purposeexpresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cellsDepositorInsertNEDD4L (NEDD4L Human)
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alanine, tryptophan 453…PromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-4Glu-iRFP670
Plasmid#167588PurposeExpresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670DepositorInsertCPSF6-4Glu-iRFP670 (CPSF6 Human)
UseLentiviralTagsiRFP670ExpressionMutationK547E, R549E, R559E, R561EPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
pUC57mini ZM249M P90A _env eGFP
Plasmid#132939PurposepUC57mini ZM249M _env eGFP containing P90A mutant capsid sequenceDepositorInsertHIV-1ZM249M provirus (P90A) with env deletion, GFP
UseLentiviralTagsExpressionMutationP90APromoterAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV 197 E7
Plasmid#85030PurposeHPV 197 E7 in CMVDepositorInsertHPV 197 E7
UseTagsExpressionMammalianMutationCodon optimized for expressionPromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-I-LaminB
Plasmid#98831PurposeIn vivo visualization of the nuclear envelope (can be used for colocalization studies)DepositorArticleInsertCytERM
UseTagsmScarlet-IExpressionMammalianMutation*(see below)PromoterCMVAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only