Addgene

148,416 results

Showing: 2661 - 2680 of 148416 results

Results per page:

Sort results by:

spisPink chromoprotein

Plasmid

#117839

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, spisPink

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-PR rep/cap

Plasmid

#197565

Purpose

encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

Depositor

Casey Maguire

Article

Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

Inserts

AAV Rep genes
AAV9 VP1 Cap gene with PR insert

Use

AAV

Tags

Expression

Mutation

contains 7mer insert PRPPSTH between amino acids …

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Use

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-puro-N-3HA-SREBP2-C-Flag

Plasmid

#134287

Purpose

Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

3x HA and Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-HMBG1

Plasmid

#215130

Purpose

Expresses mCherry-HMBG1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

HMGB1 (HMGB1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA3-Pansio

Plasmid

#214445

Purpose

Expresses eSpCas9 and gRNA targeting Pansio-1 GSH

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

eSpCas9-P2A-mRuby2-P2A-tp53dn

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA3-Olônne

Plasmid

#214447

Purpose

Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

eSpCas9-P2A-mRuby2-P2A-tp53dn

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA10.53-Clover

Plasmid

#214450

Purpose

BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

mClover

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA3-Keppel

Plasmid

#214446

Purpose

Expresses eSpCas9 and gRNA targeting Keppel-19 GSH

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

eSpCas9-P2A-mRuby2-P2A-tp53dn

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA10.5-Clover

Plasmid

#214449

Purpose

BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

mClover

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA4.721-Olônne

Plasmid

#214443

Purpose

BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA4.721-Keppel

Plasmid

#214444

Purpose

BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA4.721-Pansio

Plasmid

#214441

Purpose

BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Mar 1. ():.

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAS9-mCherry-Frame +2

Plasmid

#66941

Purpose

CRISPaint frame selector +2

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTK-DN1

Plasmid

#180282

Purpose

Cas9 cassette (TU1)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS - pPGK1 - Cas9 - tPGK1 - ConR1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pPGK1

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
gfasPurple chromoprotein

Plasmid

#117849

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, gfasPurple

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAS9-mCherry-Frame +0

Plasmid

#66939

Purpose

CRISPaint frame selector +0

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +0

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge