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Showing: 2621 - 2640 of 24275 results

  1. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Use
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

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  6. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

    Plasmid
    #115518
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    eGFP
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    115518-plasmid-map-sequence-id-229325

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  8. pTT5-hSDC1-N20

    Plasmid
    #52355
    Purpose
    expresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of SDC1 aa2-21 (according to numbering i…
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. SARS CoV-2 HexaPro spike (bio-His)

    Plasmid
    #166856
    Purpose
    Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.
    Depositor
    Evelien Bunnik
    Article
    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d
    Insert
    SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
    Use
    Tags
    8xHis and Biotinylation site
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166856-plasmid-map-sequence-id-327668

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  11. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

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  13. pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

    Plasmid
    #179459
    Purpose
    Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)
    Depositor
    Francois St-Pierre
    Article
    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00
    Has Service
    AAV1
    Insert
    JEDI-2P-Kv
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    179459-plasmid-map-sequence-id-353727

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  16. pZ:F3-P CAGGS tdTPH-F

    Plasmid
    #112667
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    112667-plasmid-map-sequence-id-229330

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  17. HA-hDAGLa-V5

    Plasmid
    #87674
    Purpose
    Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Article
    Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do
    Insert
    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    Use
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87674-plasmid-map-sequence-id-173603

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  19. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid
    #137722
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Truncated at amino acid 1328
    Promoter
    Tight TRE promoter
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137722-plasmid-map-sequence-id-352678

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  20. pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

    Plasmid
    #153062
    Purpose
    Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)
    Depositor
    Silvia Monticelli
    Article
    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi
    Insert
    Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Mutagenesis to insert a stop codon at aa 298 in B…
    Promoter
    EF1α
    Available since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153062-plasmid-map-sequence-id-304636

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Showing: 2621 - 2640 of 24275 results