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Showing: 2621 - 2640 of 3838 results

  1. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid
    #101139
    Purpose
    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.
    Depositor
    William Kaelin
    Article
    Lu et al Science. 2014 Jan 17;343(6168):305-9. do
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    101139-plasmid-map-sequence-id-194900

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  2. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid
    #101178
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    101178-plasmid-map-sequence-id-219907

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  9. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

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  13. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Availability
    Academic Institutions and Nonprofits only
    99374-plasmid-map-sequence-id-430620

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  14. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Availability
    Academic Institutions and Nonprofits only
    99375-plasmid-map-sequence-id-191025

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  15. lentiGuide-Hygro-dTomato

    Plasmid
    #99376
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Availability
    Academic Institutions and Nonprofits only
    99376-plasmid-map-sequence-id-191027

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  19. PB-UniSAM

    Plasmid
    #99866
    Purpose
    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0
    Depositor
    Lesley Forrester
    Article
    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.
    Insert
    UniSAM-mCherry + U6-gRNA2.0
    Use
    CRISPR and Synthetic Biology; Dcas9-sam activation
    Tags
    Expression
    Mammalian
    Mutation
    BbsI sites in CDS were ablated by consensus mutat…
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    99866-plasmid-map-sequence-id-192399

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Showing: 2621 - 2640 of 3838 results