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Purposemammalian expression of murine E-caderin with mCherry fusion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE-cadherin
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Alt namecadherin 1
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SpeciesM. musculus (mouse)
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Entrez GeneCdh1 (a.k.a. ARC-1, E-cad, Ecad, L-CAM, UVO, Um)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer mCherry-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Murine E-cadherin mCherry was a gift from Alpha Yap (Addgene plasmid # 71366 ; http://n2t.net/addgene:71366 ; RRID:Addgene_71366) -
For your References section:
Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens. Han SP, Gambin Y, Gomez GA, Verma S, Giles N, Michael M, Wu SK, Guo Z, Johnston W, Sierecki E, Parton RG, Alexandrov K, Yap AS. J Biol Chem. 2014 Mar 14;289(11):7764-75. doi: 10.1074/jbc.M113.544478. Epub 2014 Jan 27. 10.1074/jbc.M113.544478 PubMed 24469447