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C1-F-tractin(delta1-6)-mCitrine

Plasmid

#155219

Purpose

F-actin bidning mutant of F-tractin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
dmBACCS2VL-IRES-dOrai-IRES-mCherry

Plasmid

#72898

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha1-850

Plasmid

#62522

Purpose

C-terminal aa851-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 851-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 851-1374

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pGP-CMV-GCaMP3-44

Plasmid

#91780

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

GENIE Project, Douglas Kim

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-44

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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dmBACCS2NS-IRES-dOrai-IRES-mCherry

Plasmid

#72896

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hPMLIII

Plasmid

#103808

Purpose

BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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ATF4 17: 5’ATF4.uORF1stopm1&2.GFP

Plasmid

#21863

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stop codons between the colinear uORF1 and uORF2 …

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pmCherry-VIM-BC2T

Plasmid

#108966

Purpose

expresses mCherry-vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

VIM (VIM Human)

Use

Tags

BC2T and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-FKBP-TPD52

Plasmid

#172455

Purpose

Expression of Tumor Protein D52 (TPD52) with N-terminal GFP-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Use

Tags

GFP-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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vsv-CenpB 1-166-Sgo1 N61I-GFP

Plasmid

#108499

Purpose

expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1, no PP2A binding N…

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-TetR

Plasmid

#103798

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-TetR (CIB1, tetR E. coli, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-GFP

Plasmid

#72897

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-GFP

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-Cx43ML-N1

Plasmid

#49860

Purpose

Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

All internal Methionines mutated to Leucine. No s…

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374

Plasmid

#62524

Purpose

N-terminal aa1-269 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-269 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS302D

Plasmid

#62529

Purpose

Serine 302 of Drosha is mutated to aspartic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to aspartic acid (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to aspartic acid

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300E

Plasmid

#62528

Purpose

Serine 300 of Drosha is mutated to glutamic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to glutamic acid (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to glutamic acid

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-Abr R683A N795A

Plasmid

#36417

Depositor

Nora Heisterkamp, John Groffen

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

active BCR-related (ABR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R683A N795A

Promoter

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP d344-873

Plasmid

#21732

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

Insert

MAP4K3 (MAP4K3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Promoter

Available since

June 3, 2010

Availability

Academic Institutions and Nonprofits only