vsv-CenpB 1-166-Sgo1 N61I-GFP
(Plasmid #108499)

• Purpose: expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)
• Depositing Lab: Susanne Lens
• Publication: Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi: 10.1016/j.celrep.2015.03.052. Epub 2015 Apr 16.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CenpB-Shugoshin 1
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Mutation: CenpB 1-166 fusion before Sgo1, no PP2A binding N61I
• Entrez Gene: SGO1 (a.k.a. CAID, NY-BR-85, SGO, SGOL1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • VSV (N terminal on insert)
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HinDIII (unknown if destroyed)
• 3′ cloning site: HinDIII (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • SL-592 vsv-CenpB-Sgo1 N61I-EGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry