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pLVTHM-shRnd3

Plasmid

#86437

Purpose

2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

Depositor

Violaine Moreau

Article

Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

Insert

shRNA targeting RND3 (RND3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-hCYP26A1-FL- luciferase

Plasmid

#135566

Purpose

Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase

Depositor

Catharine Ross

Article

Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

Insert

Full-length promoter of human retinoic acid receptor-beta gene

Use

Tags

Expression

Mutation

Promoter

hHuman CYP26A1 gene promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A-TAZ S89A

Plasmid

#179911

Purpose

Entry vector of TAZ S89A for gateway coloning.

Depositor

Xin Chen

Article

Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

Insert

WW Domain Containing Transcription Regulator 1 (WWTR1 Human)

Use

Entry vector

Tags

3X Flag-tag

Expression

Mutation

Serine 89 mutated to Alanine

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH-TAZ S89A

Plasmid

#179910

Purpose

Construct for expressing an activated form of TAZ (encoding be gene WWTR1)

Depositor

Xin Chen

Article

Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

Insert

WW Domain Containing Transcription Regulator 1 (WWTR1 Human)

Use

Tags

3X Flag-tag

Expression

Mammalian

Mutation

Serine 89 mutated to Alanine

Promoter

EF1a

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ttr

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166133

Purpose

This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting cldn1 gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166134

Purpose

This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting firefly luciferase gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcrDNA3.1-Chimera

Plasmid

#100891

Purpose

Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-HH1713

Plasmid

#124994

Purpose

E. coli expression of recombinant H. hepaticus antigen HH_1713

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH_1713

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP-GFP-shch-TOG

Plasmid

#69113

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIX-Myc-hTIM3

Plasmid

#110893

Purpose

Expression of Myc-tagged hTIM3 at the cell surface

Depositor

Hyeryun Choe

Article

Jemielity et al PLoS Pathog. 2013 Mar;9(3):e1003232. doi

Insert

Hepatitis A virus Cellular Receptor 2 (HAVCR2) (HAVCR2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

CMV

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (del.Cyto)

Plasmid

#49209

Purpose

expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (del.Cyto) (Havcr2 Mouse)

Use

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

cytosolic domain deleted, no Tim3 signal sequence…

Promoter

Ef1a

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T2 truncation)

Plasmid

#49211

Purpose

expresses Flag tagged mouse Tim3 (T2 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T2 truncation (Havcr2 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T2 truncation ( lacks all but one tyrosine, which…

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T1 truncation)

Plasmid

#49210

Purpose

expresses Flag tagged mouse Tim3 (T1 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T1 truncation (Havcr2 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T1 truncation ( contains all but the three most C…

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG

Plasmid

#69114

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

L1939A and L1942A amino acid substitutions. Sile…

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-a

Plasmid

#17590

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-a (SPP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 49–942

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pGCDNsam-Foxa3

Plasmid

#33005

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A3 (Foxa3 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pDest490-OPN-c

Plasmid

#17592

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-c (SPP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–93, 175–942

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pJBM4

Plasmid

#225291

Purpose

E. coli-Campylobacter shuttle vector; TetR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Tet(O) and its putative promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa3-IRES-GFP

Plasmid

#33009

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A3 (Foxa3 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only