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Plasmid#87102Purposeexpresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosaDepositorInsertpchA WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Alpha
Plasmid#177961PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)DepositorInsertSARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
UseTagsExpressionMammalianMutationdel69, 70, del144, D614G, N501Y, A570D, P681H, T7…PromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PKD1-F (pCI-PKD1-Flag)
Plasmid#21369DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
UseTagsFLAGExpressionMammalianMutationwild-type sequence*PromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-DIO-CB2-EGFP
Plasmid#91946PurposeExpression of mouse CB2 cannabinoid receptors and eGFP in the presence of CreDepositorInsertsEGFP
type-2 cannabinoid receptor
UseAAVTagsFLAG and NoneExpressionMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
UseTags6xHis tag and HRV3C cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS EFF-1::V5
Plasmid#133584PurposeExpresses EFF-1 A full length protein in mammalian cellsDepositorInsertEFF-1::V5
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMA3200
Plasmid#46855PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12DepositorInsertExoY
UseLentiviralTagsFKBP E31G, R71G, K105E and MycExpressionMutationPromoterTRE-TightAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_wt
Plasmid#149386PurposeP. aeruginosa PA14 CRISPR2 locusDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET sfGFP(150TAG)
Plasmid#133455Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cellsDepositorInsertsfGFP
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB BmOr2 (Y464A)
Plasmid#73927PurposeUAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to AlanineDepositorInsertBmOr2 (Y464A)
UseTagsExpressionInsectMutationchanged 464 Tyrosine to AlaninePromoterhsp70Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMA3228
Plasmid#46856PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12DepositorInsertExoY K81M
UseLentiviralTagsFKBP E31G, R71G, K105E and MycExpressionMutationPromoterTRE-TightAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-BFP
Plasmid#215720PurposeCatalytically inactive dCasRx with 2A-BFPDepositorInsertdCasRx
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS
Plasmid#196484PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterCMV (synthetase), Pol III tRNA promoter (tRNA)Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Flag-Rheb
Plasmid#165031Purposeexpression of Flag tagged Rheb protein in mammalian cellsDepositorInsertRheb (RHEB Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry-MHC-IIA
Plasmid#35687DepositorInsertMHC IIA (MYH9 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP 150TAG
Plasmid#85483PurposeArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150TAG (amino acid 150 mutated to stop codon)PromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG058
Plasmid#214261PurposePaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)DepositorInsertCore genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
UseTags6xHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcPylRS(Y384F Y306A)
Plasmid#196486PurposeTo express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.DepositorInsertpyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-UTRN-mini
Plasmid#158749PurposeExpresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cellsDepositorInsertUTRN-mini
UseTagseGFPExpressionMammalianMutationPromoterCMV IE94Available sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+1D
Plasmid#149389PurposeP. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation1bp inserted downstream of the IHFprox sitePromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only