pCAGGS EFF-1::V5
(Plasmid #133584)

• Purpose: Expresses EFF-1 A full length protein in mammalian cells
• Depositing Lab: Benjamin Podbilewicz
• Publication: Avinoam et al Science. 2011 Apr 29;332(6029):589-92. doi: 10.1126/science.1202333. Epub 2011 Mar 24.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4741
• Total vector size (bp): 6797
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EFF-1::V5
• Alt name: pOA19
• Species: C. elegans (nematode)
• Insert Size (bp): 2064
• GenBank ID: 174334
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: pCAGGS Fw
• 3′ sequencing primer: pCAGGS Rv

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mohler WA, et al. Dec Cell 2002 Podbilewicz B, et al. Dec Cell 2006

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry