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EF1a-3NLS-Sox2TALE-GCN4

Plasmid

#120548

Purpose

Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-3NLS-Klf4 TALE-GCN4

Plasmid

#120546

Purpose

Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 gene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, Klf4 TALE, GCN4 (KLF4 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEF_PE2

Plasmid

#199267

Purpose

Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Use

Tags

Expression

Mammalian

Mutation

Δ40-903

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGAMA-YAP

Plasmid

#74942

Purpose

Lentiviral over-expression vector for YAP

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

T2A-mCherry

Expression

Mutation

Promoter

EF1α

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-CDK6-mutPAM-PGK-puro

Plasmid

#187638

Purpose

sg resistant CDK6 ORF

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

PAM site mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PEmax_AAVS1_knockin_HDR_donor

Plasmid

#223000

Purpose

HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PEmax

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_R248W

Plasmid

#183114

Purpose

Expresses flag-tagged p53 with R248W mutation in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed arginine 248 to tryptophan

Promoter

EF1a

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_WT

Plasmid

#183112

Purpose

Expresses flag-tagged p53 in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes1+2 f.l. WT

Plasmid

#158540

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

Plasmid

#149714

Purpose

Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 1)

Plasmid

#191341

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GV

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 2)

Plasmid

#191342

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GV

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 2, transient)

Plasmid

#197589

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_P278A

Plasmid

#183113

Purpose

Expresses flag-tagged p53 with P278A mutation in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 278 to alanine, see depositor com…

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_R248W+P278A

Plasmid

#183115

Purpose

Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 278 to alanine and arginine 248 t…

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

Plasmid

#24419

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

RS1 (HTR4 Human)

Use

Mouse Targeting

Tags

FLAG and mCherry

Expression

Mutation

Human 5HT4B receptor with D100A mutation, generat…

Promoter

Available since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-NmClover3-hsLem2

Plasmid

#179524

Purpose

Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

LEMD2 (LEMD2 Human)

Use

Tags

mClover3-mAID

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

Plasmid

#225708

Purpose

Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5-Kv2.1

Use

AAV

Tags

Expression

Mutation

Promoter

EF1α

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes 1-480 WT

Plasmid

#158541

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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