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Plasmid#111762PurposeExpresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS9-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS7
Plasmid#111761PurposeExpresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS7-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS5
Plasmid#111760PurposeExpresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS5-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS7
Plasmid#111764PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS9
Plasmid#111765PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-26
Plasmid#120005PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mAID-CloverDepositorInsertmAID-Clover
UsePcr templateTagsmAID-CloverExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS5
Plasmid#111763PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P26
Plasmid#120037PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-CloverDepositorInsertmAID-Clover
UsePcr templateTagsmAID-CloverExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z26
Plasmid#120069PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-CloverDepositorInsertmAID-Clover
UsePcr templateTagsmAID-CloverExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWKS10
Plasmid#139417PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.DepositorInsertClover
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-FUCCI
Plasmid#133363PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporterDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsHA-tagged Clover-GemininExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG FUCCI
Plasmid#136934Purposedonor plasmid for constitutive FUCCI expression in human cellsDepositorInsertClover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
UseSynthetic BiologyTagsClover and mKO2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-FUCCI-Luc
Plasmid#133364PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporterDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsHA-tagged Clover-GemininExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
ER2-mGreenLantern
Plasmid#186548PurposeLocalization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiencyDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.244
Plasmid#73756PurposeNabi2.244 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.244
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.242
Plasmid#73754PurposeNabi2.242 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.242
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.30
Plasmid#73798PurposeNabi2.30 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.30
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.104
Plasmid#73799PurposeNabi2.104 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.104
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.125
Plasmid#73800PurposeNabi2.125 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.125
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.216
Plasmid#73801PurposeNabi2.216 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.216
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only