SOX2-P2A-NLS-mClover3-IRES-Puro
(Plasmid
#215544)
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PurposeDonor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSOX2 homologous recombination arms with IRES-Puro selection cassette
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SpeciesH. sapiens (human)
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Insert Size (bp)1584
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Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SOX2-P2A-NLS-mClover3-IRES-Puro was a gift from Kai Wang (Addgene plasmid # 215544 ; http://n2t.net/addgene:215544 ; RRID:Addgene_215544)