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  1. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted... in titer results between ddPCR and qPCR? A: At CLOVER, we have traditionally used qPCR (protocol link...dense labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg per mouse to get...

  2. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated our submission...Data Hub If you've ever validated an Addgene or CLOVER AAV, you can (and dare we say, should!) submit ...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with...Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible...

  5. Summer Fun at Addgene!

    Type
    Blog Post
    ...Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite you to head on over...

  6. Caltech Systemic Capsids

    Type
    Collection
    ...the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in a new window) . As part of...The following decision tree was developed at the CLOVER Center at Caltech and can help guide your decision...Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center (Link opens in a new window) and was adapted...please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository of published capsid use (Link...

  7. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with...Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression Clover 505 515 84 6.1 30 min Prone to dimerization pcDNA3-Clover - Mammalian Expression...Expression pFA6a-link-yoClover-Kan - Yeast Expression dClover2 505 515 Dimer (A206K) dClover2-N1 - Mammalian ...Expression dClover2-C1 - Mammalian Expression mClover3 506 518 85 6.5 Monomer (A206K) pNCS-mClover3 - Bacterial...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...experiment? Look it up in Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly...more options) we recommend you check out Caltech’s CLOVER Center’s extensive repository of published systemic... on reagents and protocols, visit https://clover.caltech.edu. Figure adapted from Challis et al. 2022....

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's, FTD Michael Davidson 56371 Clover-MAPTau-C-10 MAPT Clover CMV Parkinson's, FTD Michael Davidson...43ΔNLS-mClover3 TARDBP mClover3 TRE ALS Ophir Shalem 214917 TRE-mClover3-TDP-43ΔNLS TARDBP mClover3 TRE ...mScarlet CAG ALS Beverly Koller 118760 VPS13C^mClover3 VPS13C mClover3 inserted at position aa 1914 CMV Parkinsonism...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...versions of the mAID vectors come with mCherry2 or mClover fluorescent proteins allowing you to screen for...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ...). pTALYM3 and pTALYM4 contain TALE fused with mClover and mRuby2, respectively, and can be used as destination...
Showing: 1 - 15 of 15 results