1. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pX330-sgRNA_Drosha_3

   Plasmid

   #79862

   Purpose

   CRISPR/Cas9 DROSHA

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   sgRNA Drosha
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

2. Cdk12_intron3_sg2_pX330

   Plasmid

   #127174

   Purpose

   sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-mcherry

   Plasmid

   #98750

   Purpose

   Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

   Depositor

   Jinsong Li

   Article

   Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-SpCas9-HF1

   Plasmid

   #108301

   Purpose

   pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330-HypaCas9

   Plasmid

   #108302

   Purpose

   pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330-sgRNA_Dgcr8_1

   Plasmid

   #73525

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pX330-sgRNA_Dgcr8_3

   Plasmid

   #73527

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pX330-sgRNA_Dgcr8_4

   Plasmid

   #73528

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pX330-sgRNA_Dgcr8_2

   Plasmid

   #73526

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pX330-TJP1

    Plasmid

    #227299

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA TR KO 2

    Plasmid

    #207610

    Purpose

    sgRNA 2 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    TCAGGCCGCAGGAAGAGGAA
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgRNA TR KO 1

    Plasmid

    #207609

    Purpose

    sgRNA 1 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    ACCCTAACTGAGAAGGGCGT
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330-EN1201

    Plasmid

    #92144

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330-EN479

    Plasmid

    #86234

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse rosa26 locus
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKC123

    Plasmid

    #134240

    Purpose

    expresses gRNA targeting CENP A 3'

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP A 3' CRISPR construct
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330-PCNT

    Plasmid

    #227284

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of PCNT (PCNT Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3xMS2 TR knockin sgRNA 1

    Plasmid

    #207607

    Purpose

    sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    cgactcgcccggcagcgcac
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3xMS2 TR knockin sgRNA 2

    Plasmid

    #207608

    Purpose

    sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    acccccaaacctgactgact
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge