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Showing: 241 - 260 of 9801 results

  4. pSpCas9(BB)-2A-Neo

    Plasmid
    #127762
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127762-plasmid-map-sequence-id-247554

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  7. pSicoR-Ef1a-mCh

    Plasmid
    #31847
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA; Ef1alpha for mCherry
    Available since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31847-plasmid-map-sequence-id-35883

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  9. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  14. dCas9 plasmid

    Plasmid
    #100091
    Purpose
    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3XFlag-NLS-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100091-plasmid-map-sequence-id-197565

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  15. pCDH-EF1

    Plasmid
    #72266
    Purpose
    Express gene of interest under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72266-plasmid-map-sequence-id-447561

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  16. MSCV-IRES-GFP

    Plasmid
    #20672
    Purpose
    Depositor
    Tannishtha Reya
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20672-plasmid-map-sequence-id-203241

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  18. pSBbi-RB

    Plasmid
    #60522
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60522-plasmid-map-sequence-id-97524

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  19. TLCV2-DelGFP

    Plasmid
    #133302
    Purpose
    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.
    Depositor
    Jingshi Shen
    Article
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133302-plasmid-map-sequence-id-271497

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  20. pX333

    Plasmid
    #64073
    Purpose
    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    3XFLAG-Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CBh; U6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64073-plasmid-map-sequence-id-360030

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Showing: 241 - 260 of 9801 results