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Showing: 601 - 650 of 4441 results

  1. pUC57-mini v2-HF24_F23

    Plasmid
    #195737
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#23 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195737-plasmid-map-sequence-id-385577

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  2. pUC57-mini v2-HF24_F21

    Plasmid
    #195735
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#21 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195735-plasmid-map-sequence-id-385575

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  3. pUCP20 PilB113*Q119A

    Plasmid
    #162867
    Purpose
    Expression of PilB Amber with a C-terminal histag
    Depositor
    Katrina Forest
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    Q113Amber, Q119A
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162867-plasmid-map-sequence-id-329259

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  4. pUCP20 PilB113*L197D

    Plasmid
    #162868
    Purpose
    Expression of PilB Amber with a C-terminal histag
    Depositor
    Katrina Forest
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    Q113Amber, L193D
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162868-plasmid-map-sequence-id-329261

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  7. Rp51a_10bp 5'SS_No BP or pBP_pUC18

    Plasmid
    #100989
    Purpose
    Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Aaron Hoskins
    Article
    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1
    Insert
    RP51A (RPS17A Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    100989-plasmid-map-sequence-id-194327

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  8. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

    Plasmid
    #100990
    Purpose
    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Aaron Hoskins
    Article
    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1
    Insert
    RP51A (RPS17A Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    100990-plasmid-map-sequence-id-194330

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  10. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid
    #181748
    Purpose
    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Type
    Empty backbone
    Use
    Substrate plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181748-plasmid-map-sequence-id-354677

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  11. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  20. pUC19-hsg(G:H)

    Plasmid
    #138259
    Purpose
    Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Insert
    mCherry targeting sgRNAs G and H
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    138259-plasmid-map-sequence-id-273638

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  40. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175004-plasmid-map-sequence-id-347131

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  41. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  47. pUCmini-iCAP-AAV.CAP-B22

    Plasmid
    #175005
    Purpose
    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175005-plasmid-map-sequence-id-347133

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Showing: 601 - 650 of 4441 results