pUC19-POLBHR-eGFP
(Plasmid #176064)

• Purpose: Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
• Depositing Lab: Robert Sobol
• Publication: Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi: 10.1016/j.celrep.2021.109917.

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: Christopher Koczor
• Total vector size (bp): 5137
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP-PolB
• Species: H. sapiens (human)
• Insert Size (bp): 2496
• Mutation: pUC with endogenous SapI mutated out, PAM mutation for PolB g1
• Entrez Gene: POLB
• Promoter: N/A
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pUC19-POLBHR-eGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry