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Plasmid#191646PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MoFGFR3b-SV40PA
Plasmid#86493Purposemamalian cell expression of FGFR3bDepositorInsertFgfrR3 IIIb 12F5 (Fgfr3 Mouse)
UseTagsExpressionMammalianMutationPromoterMoloney murine leukemia virus LTRAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sox2-t2A-GFP
Plasmid#127537PurposeExpresses human SOX2 and eGFP via t2A linker.DepositorInsertSOX2 (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CA-Stat3-t2A-mCherry
Plasmid#127540PurposeExpresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.DepositorInsertStat3 (Stat3 Mouse)
UseTagsExpressionMammalianMutationA662C and N664C. Residues changed to Cys renders …PromoterCMVAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS2-TVA-eGFP-N2cG
Plasmid#175439PurposeCre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoerDepositorInsertTVA-P2A-eGFP-P2A-N2cG
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sox2-t2A-mCherry
Plasmid#127538PurposeExpresses human SOX2 and mCherry via t2A linker.DepositorInsertSOX2 (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/cyto-iAspSnFR
Plasmid#201394PurposeCMV overexpression of cyto-iAspSnFR in mammalian cell linesDepositorInsertcyto-iAspSnFR
UseTagsExpressionMammalianMutationgltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…PromoterCMVAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD200-Cd4d3+4-COMP-blac-3xFLAG-6his
Plasmid#71471PurposeA positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kitDepositorInsertCD200 (Cd200 Rat)
UseTags6xHis tag, Cd4d3+4, beta-lactamase, and triple FL…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)
Plasmid#191650PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-NFIB-T2A-miRFP670
Plasmid#187222PurposeExpresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertNuclear Factor I B (NFIB Human)
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro
Plasmid#198441PurposeActivity/inhibition assays, 19F-NMRDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A,PromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS5-TVA-eGFP-N2cG
Plasmid#175440PurposeCre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoerDepositorInsertTVA-P2A-eGFP-P2A-N2cG
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA_SOX2_HDR
Plasmid#163752PurposegRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.DepositorInsertgRNA_SOX2 (SOX2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry
Plasmid#202090PurposeNeuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutationsDepositorInsertdCas9-Set2(R195G, C201A)
UseTags3x FLAG and mCherryExpressionMammalianMutationR195G and C201A abolish catalytic activityPromoterhuman SynapsinAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-Gja1prom
Plasmid#188114PurposeExpresses luciferase under control of Gja1 promoter in transfected cellsDepositorInsertGja1 promoter (Gja1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterGja1 endogenous promoter up to gene start codonAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W5F
Plasmid#198443PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W5FPromoterT7 promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
5ZiPro
Plasmid#198432PurposeConformation sensitive site specific Cys-labelingDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationS84*C, R95*A, C80S, C143S, S160C, C178S,PromoterT7 promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W61*F
Plasmid#198442PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW61*F, R95*A,PromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W5F
Plasmid#198436PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW5FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W83F
Plasmid#198439PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW83FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W89F
Plasmid#198447PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W89FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W83F
Plasmid#198446PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W83FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W69F
Plasmid#198438PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW69FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W89F
Plasmid#198440PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW89FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W69F
Plasmid#198445PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W69FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro
Plasmid#198434PurposeActivity/inhibition assays, 19F-NMRDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W61*F
Plasmid#198435PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW61*FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W50F
Plasmid#198437PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW50FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W50F
Plasmid#198444PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W50FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)
Plasmid#191651PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4DepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only