-
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterhPGK1Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3
Plasmid#159912PurposeMutagenesis of Kcnd3DepositorInsertKcnd3 (Kcnd3 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO ChETA-EYFP
Plasmid#26968PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activationDepositorHas ServiceAAV1, AAV5, and AAV9InserthChR2(E123T/H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationE123T, H134RPromoterEF1alphaAvailable sinceJan. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Ace-mNeon2-Kv2.1PR
Plasmid#195522PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-loxP-GRAB_DA2m-loxP
Plasmid#189751PurposeExcludes sensor expression in Cre-positive neuronsDepositorInsertDopamine sensor GRAB_DA2m
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus
Plasmid#139505PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV8 and AAV9Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT3.0
Plasmid#208726PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Axon-jRex-GECO
Plasmid#172714PurposeAAV transfer plasmid for neuronal expression of axon-targeted jRex-GECO.DepositorInsertAxon-jRex-GECO
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-dDIO-MCS--4x6T
Plasmid#196422PurposeEmpty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV; Dre/rox; astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3
Plasmid#159904PurposeMutagenesis of Kcnn3DepositorInsertKcnn3 (Kcnn3 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Chronos-tdTomato]
Plasmid#84484PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-ChrimsonR-tdTomato
Plasmid#171027PurposeExpresses ChrimsonR-tdTomato fusion after Flp-dependent recombinationDepositorHas ServiceAAV1 and AAV9InsertChrimsonR-tdTomato
UseAAVTagsChrimsonR is fused to tdTomatoExpressionMutationPromoterEf1aAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-FLPo-T2A-GFP
Plasmid#161766Purposeexpress FLPo and GFP under the control of human synapsin promoterDepositorInsertsFLPo
T2AEGFP
UseAAVTagsExpressionMammalianMutationPromoterhSynapsin1Available sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff
Plasmid#107700Purposein vivo voltage imagingDepositorInsertQuasAr3-P2A-CheRiff
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-mCherry-WPRE-pA
Plasmid#37083PurposeCre-dependent expression of mCherryDepositorInsertmCherry
UseAAV and Cre/Lox; Cre-onTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-GRAB-HTR6-cilia
Plasmid#189615PurposeExpresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoterDepositorInsertHTR6_GRAB
UseAAVTagsExpressionMutationPromoterSyn1Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-mCherry
Plasmid#26975PurposeAAV expression of humanized ChR2(H134R) fused to mCherry driven by CaMKIIa promoter for optogenetic excitationDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
198_pAAV-ProD1-CatCh-GFP-WPRE
Plasmid#125977PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProD1Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50472PurposeGs-coupled rM3D-IRES-mCitrine under the control of GFAP promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-tdTomato-WPRE-pGHpA
Plasmid#67527Purpose"Expresses the fluorescent protein tdTomato"DepositorInserttandem dimer tomato
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only