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pRF-HapR

Plasmid

#49360

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

HapR repressor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRF-SmcR

Plasmid

#49371

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

SmcR repressor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRF-Orf2

Plasmid

#49366

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

Orf2 repressor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-dusp6

Plasmid

#82584

Purpose

5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

FGF-responsive element of the dusp6 promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-birA-EGFP

Plasmid

#58377

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA EGFP

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-KanMx

Plasmid

#122896

Purpose

ADE2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB2375

Plasmid

#67546

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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p8267 LentiCRISPR v2 hygro sgNT-2

Plasmid

#193978

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-HphMx

Plasmid

#122895

Purpose

ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GGE077

Plasmid

#120779

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 1 (G1_RedStarII)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

G1_RedStarII

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTarKO

Plasmid

#110540

Purpose

one-step modification of chromosomal locous using targocil/tarO gene in Staphylococcus aureus

Depositor

Suzanne Walker

Article

Lee et al ACS Infect Dis. 2018 Mar 23. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Targeting vector for locus of …

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pZEN

Plasmid

#129113

Purpose

Z. rouxii episomal vector containing ClonNAT resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE ADE2-URA3

Plasmid

#122886

Purpose

ADE2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pXf20pemIK

Plasmid

#126577

Purpose

Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2224

Plasmid

#67547

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE TRP1-KanMx

Plasmid

#122902

Purpose

TRP1-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE TRP1-NatMx

Plasmid

#122903

Purpose

TRP1-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TfRΔEctoR1-mRFP-GFPnb

Plasmid

#113555

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-mRFP-GFPnb

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.AF2373

Plasmid

#11529

Depositor

Sung-Hou Kim

Article

Liu et al J Mol Biol. 2005 Nov 25. 354(2):289-303.

Insert

NAD kinase (AF2373 A. fulgidus)

Use

Tags

HisTag, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pICE ADE2-NatMx

Plasmid

#122897

Purpose

ADE2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge