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Showing: 2521 - 2540 of 4748 results

  3. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70708-plasmid-map-sequence-id-364684

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  8. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47948-plasmid-map-sequence-id-68812

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  17. pSCL.028

    Plasmid
    #184965
    Purpose
    Test effect of extending -Eco1 a1/a2 in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    Use
    Tags
    Expression
    Yeast
    Mutation
    human codon optimized RT, a1/a2 length extended t…
    Promoter
    Gal7
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184965-plasmid-map-sequence-id-376534

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  18. pKCcas9dO

    Plasmid
    #62552
    Purpose
    High efficiency Streptomyces genome editing by CRISPR/Cas9 system
    Depositor
    Weihong Jiang
    Article
    Huang et al Acta Biochim Biophys Sin (Shanghai). 201
    Inserts
    Scocas9
    sgRNA ()
    act-orf4 homology arm
    Use
    CRISPR; Bacterial genome editing
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    j23119 and ptipA
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    62552-plasmid-map-sequence-id-237466

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  19. LV-PE_CO-Mini

    Plasmid
    #190338
    Purpose
    Lentiviral vector expressing PE_CO-Mini (Prime editor with codon-optimized and truncated Reverse transcriptase)
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini, ngRNA and pegRNA for HEK3 CTTins
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    190338-plasmid-map-sequence-id-374563

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  20. pBEAST_J23101-bphC

    Plasmid
    #128137
    Purpose
    The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032
    Depositor
    Jean-Loup Faulon
    Article
    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:
    Insert
    bphC
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128137-plasmid-map-sequence-id-252649

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Showing: 2521 - 2540 of 4748 results