SQT1665
(Plasmid #78744)

• Purpose: Human expression plasmid for LbCpf1-NLS-3xHA
• Depositing Lab: Keith Joung
• Publication: Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.

Backbone

• Vector backbone: pCAG-GFP
• Backbone manufacturer: Cepko lab (Addgene plasmid # 11179)
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
• Alt name: Cpf1
• Alt name: LbCas12a
• Species: Lachnospiraceae bacterium ND2006
• Promoter: CAG
• Tag / Fusion Protein:
  • NLS-3xHA (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
• 3′ sequencing primer: M13-rev

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid expresses Cpf1 from Lachnospiraceae bacterium ND2006 in mammalian cells. Recommended to use with BPK3082 (Addgene plasmid# 78742), which can be used to express a gRNA for LbCpf1.

Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.