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Plasmid#200377PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CoChR-mRuby2-ST
Plasmid#109131PurposeCation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertCoChR-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF2
Plasmid#200379PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin wPGK-Cre
Plasmid#101242Purposelow-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expressionDepositorInsertCre recombinase
UseLentiviralTagsExpressionMutationsee attached filePromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT276
Plasmid#122607Purposeexpress CDA1-BE4max in mammalian cellsDepositorInsertCDA1-BE4max
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-Flag-CtIP-T847E
Plasmid#71110Purposemammalian expession of Flag-CtlP T847E mutationDepositorInsertCtlP
UseLentiviralTagsFlagExpressionMammalianMutationT847EPromoterTRE promoter, Tet ONAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNICE HA-mSYD1A
Plasmid#59361PurposeExpression plasmid for HA-tagged mSYD1A / Syde1 (HA-tag at N-terminus of mSYD1A)DepositorInsertmSYD1A (Syde1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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piggybac-Syn-mScn8a-3xHA
Plasmid#182555PurposeExpresses Nav1.6 tagged with 3xHADepositorInsertScn8a (Scn8a Mouse)
UseTags3xHAExpressionMammalianMutationPromoterSynAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CAG:: ChR2HA-2a-hM4D
Plasmid#52520PurposeCo-expresses HA tagged channelrhodopsin and hM4DDepositorInsertChR2-2a-hM4D
UseTags2xHAExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNICE HA-mSYD1B
Plasmid#59362PurposeExpression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)DepositorInsertmSYD1B (Syde2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/WT M87
Plasmid#89322Purposeexpression of WT M87 spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deletedPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF05
Plasmid#52558Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF05
UseTagsExpressionInsectMutationchanged from GCaMP3: D397N/G398A/N399DPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF0
Plasmid#200374PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF1
Plasmid#200375PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/WT M1
Plasmid#89321Purposeexpression of M1 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deletedPromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M1C448Y
Plasmid#89319Purposeexpression of M1 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, C448YPromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn2a-HaloTag-V5-EF1-sfGFP
Plasmid#182564PurposeFor mouse Nav1.2 knockin with HaloTag-V5 at the C-terminalDepositorInsertsmouse Scn2a gRNA and HaloTag-V5 donor DNA
sfGFP
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-PKCg-C1
Plasmid#112266PurposeITRACKgamma Donor ConstructDepositorInsertprotein kinase c gamma (Prkcg Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP/WT SP M85
Plasmid#89317PurposeExpression of mouse GFP/WT spastin M85 in mammalian cellsDepositorInsertSPAST M85 (Spast Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-PP-hNF2
Plasmid#107148Purposebacterial expressionDepositorInsertneurofibromin 2 (NF2 Human)
UseTags(His)6-ThioredoxinExpressionBacterialMutationPromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChR2-mRuby2-ST
Plasmid#109125PurposeCation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChR2-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M87 C448Y
Plasmid#89320Purposeexpression of M87 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deletedPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
UseTagsExpressionMammalianMutationPromoterEF-1a promoter and RPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH2069
Plasmid#179224PurposePnmr-1::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherryDepositorInsertUNC-9 (unc-9 Nematode)
UseTagswCherryExpressionWormMutationPromoterPnmr-1Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE9-His-p97deltaD2
Plasmid#17229DepositorInsertp97 (Vcp Mouse)
UseTagsHisExpressionBacterialMutationchanged codon 457 to stop codonPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G0-R0
Plasmid#101803PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-E1.1-NRSE-RFP
Plasmid#22929DepositorInsertE1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
FAM19A1-V5
Plasmid#184337PurposeExpression of V5-tagged mouse FAM19A1DepositorInsertFAM19A1 (Tafa1 Mouse)
UseTagsV5-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH2141
Plasmid#179248PurposePunc-4::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherryDepositorInsertUNC-9 (unc-9 Nematode)
UseTagswCherryExpressionWormMutationPromoterPunc-4Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH2037
Plasmid#179220PurposePsra-11::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherryDepositorInsertUNC-7 (unc-7 Nematode)
UseTagswCherryExpressionWormMutationPromoterPsra-11Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn2a-3xV5-EF1-smFP-flag
Plasmid#182561PurposeFor mouse Nav1.2 knockin with 3xV5 at the C-terminalDepositorInsertsmouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV1-EF1α-F-Flex-jGCaMP7b-WPR
Plasmid#171689PurposeFlp-dependent expression of jGCaMP7bDepositorInsertGCaMP7b
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nirButterfly
Plasmid#136590PurposeExpression of a genetically-encoded voltage indicator nirButterflyDepositorInsertnirButterfly
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS15
Plasmid#52652Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS15
UseTagsExpressionMammalianMutationdeleted G47/H48 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Chronos(M140T)-mRuby2-ST
Plasmid#109129PurposeModified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChronos(M140T)-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1WT
Plasmid#89327PurposeInducible expression of WT M1 spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deletedPromoterTet-responsive P tightAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 WT
Plasmid#89328PurposeInducible expression of WT M87 spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deletedPromoterTet-responsive P tightAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX28
Plasmid#46850PurposeTOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiencyDepositorInsertattB, RFP and PGK-puro
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-chNfasc (+RSLE)
Plasmid#89418PurposeTwo-hybrid systemDepositorInsertNFASC (NFASC Chicken)
UseTagsGAL4 DBDExpressionYeastMutationPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS14
Plasmid#52651Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS14
UseTagsExpressionMammalianMutationdeleted G47 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS06
Plasmid#52647Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS06
UseTagsExpressionMammalianMutationM374Q mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP-HA-DAT W63A
Plasmid#90246PurposeExpresses YFP-HA-DAT W63A mutant in mammalian cellsDepositorInsertDAT (dopamine transporter) (SLC6A3 Human)
UseTagsExpressionMammalianMutationinserted HA tag (see YFP-HA-DAT), mutated W63 to APromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-HA-DAT R60A
Plasmid#90245PurposeExpresses YFP-HA-DAT R60A mutant in mammalian cellsDepositorInsertDAT (dopamine transporter) (SLC6A3 Human)
UseTagsExpressionMammalianMutationinserted HA tag (see YFP-HA-DAT), mutated R60 to APromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVenus-PICK1-shRNA
Plasmid#72575PurposeExpresses shRNA against PICK1 as well as the marker Venus fluorescent proteinDepositorUseTagsExpressionMammalianMutationPromoterH1 promoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
6his R.3-AR GUSpET28a+/pLysS
Plasmid#61157PurposeN-terminal His tagged round 3 aldehyde-resistant GUS expression vectorDepositorInsertGUSAR
UseTags6x HisExpressionBacterialMutationN66D, D151N, A219V, I396T, T480A, Q498R, D508E an…PromoterT7Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCMV Lex VP16 (F442A) HA (P#1710)
Plasmid#14595DepositorInsertLexA
UseTagsHA and VP16 (F442A)ExpressionMammalianMutationThe VP16 sequence contains an F442A mutation. The…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS12
Plasmid#52650Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS12
UseTagsExpressionMammalianMutationdeleted R40/R41/K42 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only