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Plasmid#92308PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHumanized A. aeolicus BioID2 with R40G and MycExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only
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pSpCas9_T2A_mCherry sgAAVS1
Plasmid#192480PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as controlDepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bsd
Plasmid#214370PurposeCloning vector with BlasticidinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFLP3
Plasmid#64946PurposeFlp recombinase expression vectorDepositorInsertFlp recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBB541
Plasmid#27394DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
mH2A1.1-CT-GFP
Plasmid#45167DepositorInsertH2Afy (MACROH2A1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mClover3
Plasmid#74236PurposeConstitutive expression of mClover3 in bacteriaDepositorHas ServiceCloning Grade DNAInsertmClover3
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato
Plasmid#107738PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre, dTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-g418
Plasmid#201141PurposepLEX_307 plasmid with g418 markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV:cTNT::Luciferase
Plasmid#69915PurposeUsed to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.DepositorInsertFirefly luciferase
UseAAVTagsExpressionMutationPromoterChicken cardiac troponin TAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mScarlet
Plasmid#131001PurposeAAV vector to drive the expression of mScarlet under the control of human Synapsin promoterDepositorHas ServiceAAV1 and AAV8InsertmScarlet
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro2-H2B-iRFP
Plasmid#197352PurposeEncoding nuclear-targeted iRFP713.DepositorInsertiRFP with nucleus-targeting sequence
UseLentiviralTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Taq
Plasmid#102793PurposeExpresses Taq DNA polymerase under WT T7 promoterDepositorInsertTaq DNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_IRES_mC_pGK_tBFP
Plasmid#79123PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch ReceptorsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-BRD4-WT
Plasmid#90331Purposeexpresses Wild type BRD4DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationWild typePromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-iRFP670 BlastR
Plasmid#84385PurposeLentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsiRFP670ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-GFP
Plasmid#78548PurposeCas9 protein with GFPDepositorInsertT2A-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.iGluSnFR3.v857.GPI
Plasmid#178338PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV GFAP iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BeGFP
Plasmid#21210DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-eGFPExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c_ddCpf1_final
Plasmid#212040PurposeBacterial expression of the CRISPRi system for genome editing in E. coliDepositorInsertddCpf1
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRMC2
Plasmid#68940Purposetetracycline-inducible expression vector for Staphylococcus aureusDepositorTypeEmpty backboneUseTet inducible e. coli/staphylococcus shuttle vect…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-1x
Plasmid#172281PurposeExpressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequenceDepositorInsertEGFP-N1-1x
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-cyto-iATPSnFR1.0
Plasmid#102553Purposeexpresses cytoplasmic ATP sensor in astrocytesDepositorInsertiATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_TGFBR1_WT
Plasmid#82237PurposeGateway Donor vector containing TGFBR1 , part of the Target Accelerator Plasmid Collection.DepositorInsertTGFBR1 (TGFB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Inducible Caspex expression
Plasmid#97421PurposeFor stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomicsDepositorInsertsCaspex
T2A-Gfp
UseCRISPRTags3xFLAG and V5ExpressionMammalianMutationdCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …PromoterTRE3GAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-hMLH1dn
Plasmid#174827PurposeMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)DepositorInsertPE2-P2A-hMLH1dn
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.Cre.WPRE.hGH
Plasmid#105550PurposeAAV expression of Cre from GFAP promoterDepositorHas ServiceAAV PHP.eB and AAV5InsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterGFAPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-postASAP
Plasmid#178792PurposeExpresses voltage sensor targeted to dendrites and spinesDepositorInsertFingR.PSD95 and ASAP2f
UseTagsExpressionMammalianMutationmutations in amino acids L146G, S147T N149R, S150…PromoterCAGAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Peredox
Plasmid#73807PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)DepositorInsertPeredox
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-ApoE4
Plasmid#197148PurposeExpresses ApoE4 in Mammalian cellDepositorInsertApoE4 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI WT
Plasmid#167289PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-myc-CAR-2A-Thy1.1
Plasmid#127890PurposeExpresses CART receptor along with tags.DepositorInsertsmyc,
CAR
2A
Thy1.1
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
IgK-IFN-luc
Plasmid#14886PurposeReporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expressionDepositorInsertIgK-IFN (IFNB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Fibrillarin
Plasmid#26673DepositorInsertFibrillarin (FBL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP680-N1
Plasmid#136557PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)DepositorInsertmiRFP680
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT-Pan
Plasmid#99638PurposeEGFP-mCherry control reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCX459
Plasmid#229214PurposeCRISPR array plasmid for MAP1B (Spacers 1-24)DepositorArticleInsertCRISPR array plasmid for MAP1B (Spacers 1-24)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only