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Plasmid#101298PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 of alpha-CateninPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#92140PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.DepositorInsertAID[71-114]-eGFP
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Zeta 1 (JCE875)
Plasmid#182027Purposeyeast expression of mouse PKC Zeta isoform 1 with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Zeta isoform 1
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-HDAC3
Plasmid#63676PurposeMammalian expression of Flag tagged HDAC3DepositorInsertHDAC3 (Hdac3 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE9-His-p97(wt)
Plasmid#14666DepositorInsertp97 (Vcp Mouse)
UseTagsRGS6xHisExpressionBacterialMutationSee depositor comments below.PromoterAvailable sinceApril 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mTet2 catalytic domain HxD
Plasmid#79611PurposeExpression of catalytically inactive mouse Tet2DepositorInsertTet2 (Tet2 Mouse)
UseLentiviralTagsMycExpressionMutationMutant Tet2 with H1302Y, D1304A substitutions in …PromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 Flag JIP3b
Plasmid#53458Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pKAM-ME-Flag-BFP
Plasmid#101868PurposeLentiviral Mds1-Evi-1-Flag with dual BFP and puro markersDepositorInsertMds1-Evi-1 (Mecom Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFPc2 mAbp1
Plasmid#26719DepositorInsertActin-binding Protein-1 (Dbnl Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-C/EBPβ
Plasmid#33363DepositorInsertCebpb (Cebpb Mouse)
UseTagsFLAGExpressionMammalianMutationDominant Negative (see comments)PromoterCMVAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
PKC epsilon WT
Plasmid#21240DepositorInsertPKC epsilon (Prkce Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
Plasmid#131463PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53DepositorInsertH3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsTrp53-V5, H3F3A-EGFP-AU1ExpressionMammalianMutationH3F3A G34R, Pdgfra D842VPromoternone (4x polyA to mitigate episomal expression)Available sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF
Plasmid#201217PurposepCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTFDepositorInsertADGRL3 (Adgrl3 Mouse)
UseTagsFlag and SNAPExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK3 WT
Plasmid#12186DepositorInsertMEKK3 (Map3k3 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 mSmoothened WT
Plasmid#126408PurposeFull length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV Cbx5-V5
Plasmid#175105PurposeLentiviral expression of mouse Cbx5-V5DepositorInsertCbx5 (Cbx5 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Dcst2-3xHA
Plasmid#183543PurposeExpression vector of mouse DC-STAMP domain containing 2 (Dcst2) tagged with 3xHA at C-terminus.DepositorInsertDC-STAMP domain containing 2 (Dcst2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-Arl13b11
Plasmid#40873DepositorInsertArl13b (Arl13b Mouse)
UseTagsHis and V5ExpressionMammalianMutationno stop codonPromoterCMVAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
nCas9-PPE
Plasmid#140445PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only