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Purposebicistronic expression of RORb and EGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCBIG
- Total vector size (bp) 10187
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRORb
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Alt nameRorb
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Alt nameROR-Beta
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SpeciesM. musculus (mouse)
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Insert Size (bp)1395
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Entrez GeneRorb (a.k.a. Nr1f2, RZR-beta, RZRB, Rorbeta, hstp)
- Promoter CMV/beta-actin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HpaI (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence from the depositing laboratory. According to the depositor, these differences are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CBIG-RORb was a gift from Jeffrey Macklis (Addgene plasmid # 48709 ; http://n2t.net/addgene:48709 ; RRID:Addgene_48709)