-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 24069 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMIGR
- Backbone size w/o insert (bp) 6000
-
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameRorgt
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1500
-
GenBank IDNM_011281.2
-
Entrez GeneRorc (a.k.a. Nr1f3, RORgamma, TOR, Thor)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII/BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pLXSN 5 (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MIGR-RORgt was a gift from Dan Littman (Addgene plasmid # 24069 ; http://n2t.net/addgene:24069 ; RRID:Addgene_24069)