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Addgene
Showing: 2521 - 2540 of 3838 results
  1. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
  2. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid
    #79601
    Purpose
    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.
    Depositor
    Insert
    d2GFP-Zeb1 3'UTR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pHAGE-E-cadherin-RFP

    Plasmid
    #79603
    Purpose
    pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system
    Depositor
    Insert
    CDH1 promoter region (CDH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. EGFP-CAAX

    Plasmid
    #86056
    Purpose
    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.
    Depositor
    Insert
    Homo sapiens Harvey rat sarcoma (HRAS Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains the region from 825-857 of HRAS as a far…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. pYPQ202

    Plasmid
    #86198
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker
    Depositor
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis ubiquitin 10 (AtUbi10)
    Availability
    Academic Institutions and Nonprofits only
  6. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Availability
    Academic Institutions and Nonprofits only
  7. pLV-EGFP-Cre

    Plasmid
    #86805
    Purpose
    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter
    Depositor
    Insert
    Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP and nuclear localization signal: PKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 2521 - 2540 of 3838 results