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Plasmid#74046Purposeretroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1DepositorInserthuman Ikaros (IKZF1) (IKZF1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe K-Ras 12V
Plasmid#12544DepositorInsertK-Ras 12V (KRAS Human)
UseRetroviralTagsExpressionMammalianMutation12V constituitively activatedPromoterAvailable sinceSept. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-mTert
Plasmid#162555PurposeOverexpression of TertDepositorInsertOverexpression of Tert (Tert Mouse)
UseTagsExpressionMammalianMutationNonePromoterEF1AAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7b
Plasmid#104484PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7b
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Plasmid#71707PurposeA human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmidDepositorInserthumanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
UseCRISPRTags3xFLAG and hGEM(1/110)ExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLgw Cbx1-V5-EcoDam
Plasmid#59211PurposeMammalian DamID positive control lentiviral plasmid. Expresses Cbx1-V5-Dam.DepositorInsertCbx1 (Cbx1 Mouse)
UseLentiviral; DamidTagsDam and V5ExpressionMammalianMutationPromoterHeat Shock Minimal Promoter and heat shock minima…Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse Alpha2 delta-2 pMT2
Plasmid#58732Purposeexpression of mouse Alpha2 delta-2 in mammalian cellsDepositorInsertalpha2 delta-2 (Cacna2d2 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-MKK6-EE p38alpha
Plasmid#82719PurposeExpresses constitutively active human MKK6 and murine p38 alpha.DepositorUseTagsExpressionBacterialMutationSer207 to Glu, Thr211 to GluPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-hHelios-IRES-GFP
Plasmid#74047Purposeretroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1DepositorInserthuman Helios (IKZF2) (IKZF2 )
UseRetroviralTagsFLAGExpressionMammalianMutationencodes shorter version of human HeliosPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-PGK-Chst3
Plasmid#193024PurposeAAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoterDepositorInsertChst3 (Chst3 Mouse)
UseAAVTags3xFLAG and myc-tagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Farnesyl-5
Plasmid#56159PurposeLocalization: Plasma Membrane, Excitation: 604, Emission: 659DepositorInsertFarnesyl (HRAS Human)
UseTagsmCardinalExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCardinal-Actin-C-18
Plasmid#56151PurposeLocalization: Actin, Excitation: 604, Emission: 659DepositorInsertNM_001101.3 (ACTB Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCardinal-LaminB1-10
Plasmid#56165PurposeLocalization: Nuclear Envelope, Excitation: 604, Emission: 659DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCardinal-MCAK-C-7
Plasmid#56168PurposeLocalization: Microtubules/Kinesin, Excitation: 604, Emission: 659DepositorInsertMCAK (KIF2C Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28b-Ets1-ETS
Plasmid#85735PurposeExpresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440DepositorInsertDNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Mouse, Human)
UseTags6xHis following thrombin cleavage site LVPR|GExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Mcl-1-pLVX
Plasmid#166752PurposeExpress mCerulean3 fused to the N-terminus of Bcl-2 family protein, Mcl-1, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.DepositorInsertMcl-1 (MCL1 Human)
UseLentiviralTagsmCerulean3ExpressionMutationPromoterCMVAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-H1-10
Plasmid#56161PurposeLocalization: Nucleus/Histones, Excitation: 604, Emission: 659DepositorInsertH1 (H1f0 Mouse)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDule-3-nitroTyrosine (A7)
Plasmid#174078PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only