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pCMV-BLZinCh-P40

Plasmid

#188057

Purpose

Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P40

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P50

Plasmid

#188058

Purpose

Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P50

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P20

Plasmid

#188055

Purpose

Expression of BLZinCh-P20, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P20

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P30

Plasmid

#188056

Purpose

Expression of BLZinCh-P30, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P30

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLP-LNCX-PIK3CA-W11STOP

Plasmid

#25634

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pEYFP-Mitotrap

Plasmid

#46942

Purpose

Expresses Mitotrap in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Mitotrap (MTOR Human, Budding Yeast, Aequorea victoria, Synthetic)

Use

Tags

This is a fusion between a yeast mitochondrial ta…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap1g1-FKBP

Plasmid

#46945

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap1g1-FKBP

Use

Tags

Mainly mouse Ap1g1 cDNA, with a fragment of brain…

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pSLQ1653_sgSOD1

Plasmid

#63711

Purpose

Express sgSOD1 in mammalian cells of human.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

sgSOD1, CAG promoter, mCherry, puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ1371_sgNanog

Plasmid

#63710

Purpose

Express sgNanog in mammalian cells of mice.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

sgNanog, CMV promoter, puro, mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLOVE-Sox2

Plasmid

#15953

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Promoter

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Klf4

Plasmid

#19764

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAK beta

Plasmid

#141195

Purpose

Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 isoform 2 (MAP3K20 Human)

Use

Tags

Strep, HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB1alpha2_KanR_BastaR_Rb7_GFP

Plasmid

#186427

Purpose

optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Inserts

KanR
BastaR
Rb7
eGFP

Use

Synthetic Biology; Binary vector for escherichia …

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

CaMV 35S and Pnos

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS/EG.5.1

Plasmid

#218668

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG.5.1 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pαH-S-GSAS/EG.5.1 (S )

Use

Tags

HRV 3C cleavage site (before tags) C terminal on …

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

CMV

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS/EG.5

Plasmid

#212990

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pαH-S-GSAS/EG.5 (S )

Use

Tags

HRV 3C cleavage site (before tags) C terminal on …

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCBFRE-luc

Plasmid

#26897

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only
PB-NES-ZapCV2-Zinc dead

Plasmid

#203850

Purpose

Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

Depositor

Amy Palmer

Article

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Use

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Limk1

Plasmid

#163414

Purpose

Expresses LIMK1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Limk1 (Limk1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin S3A

Plasmid

#163604

Purpose

Expresses constitutively active cofilin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S3A substitution to make cofilin constitutively a…

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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