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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLOVE
- Backbone size w/o insert (bp) 8263
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsExpand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSRY (sex determining region Y)-box 2
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Alt nameSox2
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SpeciesM. musculus (mouse)
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Insert Size (bp)2457
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MutationThe recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Sox2 is ~9264 bp.
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer V5 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC NGS analysis identifies an IS4-like element at nucleotides 6946-8283 in the plasmid backbone. It is unknown what, if any, impact this may have on plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLOVE-Sox2 was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15953 ; http://n2t.net/addgene:15953 ; RRID:Addgene_15953) -
For your References section:
Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Cell Stem Cell. 2007 Sep 13. ():. 10.1016/j.stem.2007.08.008 PubMed 18371358