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Plasmid#63702PurposeInducible expression of GFP-Mps1 fusion in mammalian cellsDepositorInsertMps1 (TTK Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)PromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSI2-DCD
Plasmid#166968PurposeExpress the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADARDepositorInsertMSI2-DCD
UseRetroviralTagsMSI2-DCDExpressionMammalianMutationN495SPromoterMSCVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLTC_Clathrin propeller
Plasmid#178471PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLTC_Clathrin propeller (CLTC Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-Nix
Plasmid#100770PurposeLentiviral expression of shRNA targeting NixDepositorInsertLenti-sh-Nix
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorUseTagsGSTExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLpA-DN.JNK1
Plasmid#51942PurposeAdenoviral vector for dominant negative JNK1 expressionDepositorInsertDN JNK1 (Mapk8 Mouse)
UseAdenoviralTagsFLAGExpressionMutationdominant negative: the dual phosphorylation moti…PromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CMYC T58A
Plasmid#53178PurposeExpresses mouse CMYC T58A from puromycin resistance retroviral vectorDepositorInsertCMYC (Myc Mouse)
UseRetroviralTagsExpressionMammalianMutationT58APromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-nSpCas9-NG
Plasmid#171692PurposeExpresses nSpCas9-NG in mammalian cellsDepositorInsertnSpCas9-NG
UseCRISPRTagsExpressionMammalianMutationD10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…PromoterEF1aAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP KNL1 wt hardened
Plasmid#45223DepositorInsertGFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)
UseTagsTEV S15ExpressionMutationE172GPromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse c-MycV394D-IRES-RFP
Plasmid#73573PurposeMammalian retroviral expression of mouse Myc V394D mutantDepositorInsertcMyc (Myc Mouse)
UseRetroviralTagsIRES-RFPExpressionMammalianMutationV394DPromoterMSCVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
J-sRNA-331Bb
Plasmid#78285PurposeAn inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNADepositorInsertj-sRNA-331
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
p7135 pHAGE-P-CMVt-N-HA-GAW-EIF3E
Plasmid#100147PurposeExpresses EIF3EDepositorInsertEIF3E (EIF3E Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Beclin-BATS
Plasmid#30498DepositorUseTags3xFlag and hrGFPExpressionMammalianMutationBeclin 1 + 413-492 aa of BarkorPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S197A
Plasmid#131522PurposeExpresses MKP-3 with S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 197 to AlaninePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-cMyc
Plasmid#136614PurposeDox-inducible lentiviral vector expressing mouse cMycDepositorInsertcMyc (Myc Mouse)
UseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3502
Plasmid#144978PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertVENTX (VENTX Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-yHS1-M7A
Plasmid#159164PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1-M7A
UseTagsCOX4 pre-sequenceExpressionYeastMutationMutated Met 7 of the cyt b562 module of mito-yHS1…PromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-SasgRNA scaffold-CMV-nSaCas9
Plasmid#171695PurposeExpresses nSaCas9 in mammalian cellsDepositorInsertsgRNA scaffold-nSaCas9-NLS
UseCRISPRTagsExpressionMammalianMutationD10APromoterU6 and CMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ165
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only